Hi There,
I recently came across a new term "phasing". What does it mean? I only know it means "genes separated by chromosome" only, can anyone please explain it for me.
Thanks in advance.
Following are the steps, as suggested by @mira_talk
1. Reference sequences loaded and transformed into contigs, each contig containing exactly one read. 2. Sequence of each contig is cut into overlapping pieces (called rails). Length of rails: 2*...
MIRA follows for the ref based assembly (like mapping the reads on the reference with xxx, etc.) and if its possible to have a figure with the workflow.
This webpage lists some of the one-liners that we frequently use in metagenomic analyses. You can click on the following links to browse through different topics. You can copy/paste the commands as they are in your terminal screen, provided you...
USEARCH >Extreme high-throughput sequence analysis. Orders of magnitude faster than BLAST. MUSCLE >Multiple sequence alignment. Faster and more accurate than CLUSTALW.
UPARSE >OTU clustering for 16S and other marker genes....