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Wtdbg2: a de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore
By Neel 2228 days agogithub.com - Wtdbg2 is a de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore Technologies (ONT). It assembles raw reads without error correction and then builds the consensus from intermediate assembly output. Wtdbg2...Scaffolding of a bacterial genome using MinION nanopore sequencing
By Rahul Agarwal 3428 days agowww.nature.com - Second generation sequencing has revolutionized genomic studies. However, most genomes contain repeated DNA elements that are longer than the read lengths achievable with typical sequencers, so the genomic order of several generated contigs cannot...NGMLR: long-read mapper designed to align PacBio or Oxford Nanopore
By Jit 2405 days ago Comments (1)github.com - CoNvex Gap-cost alignMents for Long Reads (ngmlr) is a long-read mapper designed to sensitively align PacBilo or Oxford Nanopore to (large) reference genomes. It was designed to quickly and correctly align the reads, including those spanning...Pacasus: Correction of palindromes in long reads from PacBio and Nanopore
By BioStar 2204 days agogithub.com - Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source...Deepbinner: a signal-level demultiplexer for Oxford Nanopore reads
By Jit 1749 days agogithub.com - Deepbinner is a tool for demultiplexing barcoded Oxford Nanopore sequencing reads. It does this with a deep convolutional neural network classifier, using many of the architectural advances that have proven successful in image classification. Unlike...Nanopolis: polish a genome assembly
By Rahul Nayak 2313 days ago Comments (1)github.com - Software package for signal-level analysis of Oxford Nanopore sequencing data. Nanopolish can calculate an improved consensus sequence for a draft genome assembly, detect base modifications, call SNPs and indels with respect to a reference genome...Live Webinar on RNA-Seq Data Analysis on 9 Nov 2016
By Strand 2958 days agoLive Webinar on RNA-Seq Data Analysis Abstract: Strand NGS supports an extensive workflow for the analysis and visualization of RNA-Seq data. The workflow includes Transcriptome / Genome alignment, Differential expression analysis with Statistical...
SPAdes hybrid genome assembly
Last updated 2554 days ago by Jit Comments (1)When you have both Illumina and Nanopore data, then SPAdes remains a good option for hybrid assembly - SPAdes was used to produce the B fragilis assembly by Mick Watson’s group. Again, running spades.py will show you the...Flye: Fast and accurate de novo assembler for single molecule sequencing reads
By Jit 2395 days agogithub.com - Flye is a de novo assembler for long and noisy reads, such as those produced by PacBio and Oxford Nanopore Technologies. The algorithm uses an A-Bruijn graph to find the overlaps between reads and does not require them to be error-corrected. After...Now time is come to revolutionize amino acid sequencing by Nanopore technology
By Rahul Agarwal 3884 days agowww.eurekalert.org - Amino acid sequencing by Nanopore recognition tunneling method
