BioStar
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RNA-seq Analysis Workshop Course Materials
By Jit 2334 days agochagall.med.cornell.edu - RNAseq can be roughly divided into two "types": Reference genome-based - an assembled genome exists for a species for which an RNAseq experiment is performed. It allows reads to be aligned against the reference genome and significantly improves...RNA-seq workflow: gene-level exploratory analysis and differential expression
By Jit 1140 days agomaster.bioconductor.org - Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were quantified to the reference transcripts, and prepare gene-level count...
Pathway Analysis
Last updated 3697 days ago by Rahul Agarwal Comments (2)Pathway Analysis is usually performed with aim to enrich the genes with their functional information and reveal the underlying biological mechanisms pursue by genes. Pathway Analysis is not only limited to what biological pathways a particular set...Integrative RNA and ChIP-Seq analysis of regulatory T-cells
By Strand 3399 days agoIntegrative RNA and ChIP-Seq analysis of regulatory T-cellsWebinar on Unique Molecular Identifier (UMI)-powered Ultra-sensitive Variant Calling using Strand...
By Strand 2573 days ago Comments (1)Webinar on Unique Molecular Identifier-powered Ultra-sensitive Variant Calling using Strand NGS - Case Study by Dr. Pandurang Kolekar, Bioinformatics Engineer, Strand Life Sciences Abstract: Unique Molecular Identifiers (UMIs) are short...RNA-Seq differential expression work flow using DESeq2
By BioStar 1187 days agowww.sthda.com - One of the aim of RNAseq data analysis is the detection of differentially expressed genes. The package DESeq2 provides methods to test for differential expression analysis.transrate: Understanding your transcriptome assembly
By Neel 2324 days agohibberdlab.com - Transrate is software for de-novo transcriptome assembly quality analysis. It examines your assembly in detail and compares it to experimental evidence such as the sequencing reads, reporting quality scores for contigs and assemblies. This...Troyanskaya Lab
The goal of our research is to interpret and distill this complexity through accurate analysis and modeling of molecular pathways, particularly those in which malfunctions lead to the manifestation of disease. We are inventing integrative methods...DADA2: Fast and accurate sample inference from amplicon data with single-nucleotide resolution
By Jit 1473 days agobenjjneb.github.io - The DADA2 tutorial goes through a typical workflow for paired end Illumina Miseq data: raw amplicon sequencing data is processed into the table of exact amplicon sequence variants (ASVs) present in each sample. The DADA2...Apollo: A Sequencing-Technology-Independent, Scalable, and Accurate Assembly Polishing Algorithm
By BioStar 1712 days agogithub.com - Apollo is an assembly polishing algorithm that attempts to correct the errors in an assembly. It can take multiple set of reads in a single run and polish the assemblies of genomes of any size. Described by Firtina et al. (preliminary version...
