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2176 days ago
CANU genome assembly parameters !
Choose the appropriate parameters to run Canu and run it. The assembly will take about an hour. You can use two cores (parameter -maxThreads=2) and you would like to disable clu...1988 days ago
List of tools frequently used while genome assembly
List of tools frequently used while genome assembly: I have used the following assemblers Spades (v. 3.10.1) CANU (v. 1.6) Unicycler (v. v0.4.1) Miniasm (v...1973 days ago
1878 days ago
Software for genome assembly !
List of bioinformatics tools/Software Website References for genome assembly: 1 Falcon https://github.com/PacificBiosciences/pb-assembly 2 Canu assembler http://canu.readthedo...1387 days ago
Bioinformatics tools for telomere to telomere assembly !
● Merfin – k-mer-based assembly and variant calling evaluation for improved consensus accuracy (Arang Rhie)● PanGenie – algorithm that leverages a pan...1034 days ago
Protocol for De novo Genome Assembly using Illumina Reads
In this protocol, we address and describe the de novo assembly method for small to medium-sized genomes. What is de novo genome assembly?The method of taking a large number of short...1247 days ago
Frequently used bioinformatics tools for viral genome analysis !
IVA: accurate de novo assembly of RNA virus genomes. Hunt M, Gall A, Ong SH, Brener J, Ferns B, Goulder P, Nastouli E, Keane JA, Kellam P, Otto TD. Bioinformatics. 2015 Jul 15;31(14)...1090 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignment (Bowtie 2)...920 days ago