Bash script to alignment of short reads against reference genome !
bwa mem -t 40 -R '@RG\tID:K12\tSM:K12' \ E.coli_K12_MG1655.fa SRR1770413_1.fastq.gz...major change from bwa mem is that we'll tell it we're working with short read data using -ax sr:...1599 days ago
Bash commandline to install Anaconda !
#The line begins with $ are the commands $...w be installed into this location: /data/sata_data/workshop/wsu29/anaconda3...y37_0 ... installing: importlib_metadata-0.8-py37_0 ... installing: j...py37_0 ... installing: pytest-remotedata-0.3.1-py37_0 ... installing:...1595 days ago
Bash command to install Miniconda !
(base) [wsu29@bladeamd-2 tmp]$ wget https://repo.anaconda.co...onda3 will now be installed into this location: /data/sata_data/workshop/wsu29/miniconda3...onda init? [yes|no] [no] >>> yes no change /data/sata_data/workshop/wsu29/mini...1595 days ago
Bash command to install bwa, samtools, picard !
...l bwa Collecting package metadata (repodata.json): done Solving environm...# environment location: /data/sata_data/workshop/wsu29/anac...tools Collecting package metadata (repodata.json): done Solvin...icard Collecting package metadata (repodata.json): done Solvin...1595 days ago
Bash command to install GATK, Bedtools and SnpEff !
...gatk Collecting package metadata (repodata.json): done Solving environm...# environment location: /data/sata_data/workshop/wsu29/anac...nsi-0.4.0-r36h96ca727_0 r-gdata pkgs/r/noarch::r-g...################# | 100% r-gdata-2.18.0 | 1.1 MB | ##...1595 days ago
Installing ggplot2 and its dependencies on Ubuntu !
jit@jit-HP-Pro-3335-MT:~/Downloads/MitoHunter/mi...checking for local ICUDT_DIR... icu61/data checking for gcc... gcc -std...set... no checking for available ICU data library (ucnv, uloc, utrans)....6_64-linux-gnu -licui18n -licuuc -licudata -L/usr/lib/R/lib -lR install...1590 days ago
1586 days ago
To convert just one specific read group to fastq
# Stop script on error. set -uex # The SRR BioProject number for the sequencing data. PROJECT=PRJNA257197 # The number of datasets to subselect from the pro...RR | head -$N > selected.txt # Store the data in the reads folder. mkdir -...1582 days ago
1581 days ago
Install python-numpy python-scipy python-matplotlib on Ubuntu !
$MT:~/Downloads/chaos$ sudo apt-get inst...ecorator python-matplotlib-data python-olefile python-pil pyt...-matplotlib python-matplotlib-data python-numpy python-olefile p...verse amd64 python-matplotlib-data all 2.1.1-2ubuntu3 [3,774 kB]...ted package python-matplotlib-data. Preparing to unpack .../09-...1581 days ago