Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignmen...924 days ago
2607 days ago
PuffAligner: a fast, efficient and accurate aligner based on the Pufferfish index
...urate and versatile aligner built on top of the Pufferfish index. PuffAligner is able to produce highly sensitive alignments, similar to those of Bowtie2, but much more quickly. While...792 days ago
Bioinformatics approach to Boar Taint
...eq2000 to >10X average coverage. Short reads generated from these samples mapped to Sus Scrofa version 10.2 reference assembly using Bowtie2. Alignment file then used for...3836 days ago
Frequent Paired-end reads (PE 2x100) mapping command lines
bowtie2 -x hs37m -X 650 -q -1 r1.fq -2 r2.fq -S r12.bowtie2.sam bwa aln hs37m.fa r1.fq > r1.sai && bwa aln hs37m.fa r2.fq > r2.sai \ && bwa sampe hs37m r1.sai r...2229 days ago
Frequent parameters for bioinformatics tools !
...0 --min_mean_q 85 --min_window_q 65 FastQ Screen --aligner bowtie2' (bwa for PacBio) --subset 1...VF) -culling_limit 2 RGI (ABR) --input_type contig bowtie2 (mapping) --sensitive  ...1332 days ago
2256 days ago
2174 days ago