1154 days ago
Comment on "REAPR: a universal tool for genome assembly evaluation"
Reapr is a tool trying to find explicit errors in the assembly based on incongruently mapped reads. It is heavily based on too low span coverage, or reads mapping to...1389 days ago
2151 days ago
Comment on "Running Trinity on RNA-seq !"
Once your assembly is complete, there are several analyses you will likely want to pursue to explore aspects of the biology of your organism based on your assembled trans...2175 days ago
Comment on "Run miniasm assembler on nanopore reads !"
I followed this to assemble the genome using ONT (nanopore reads) Minimap and miniasm are ultrafast tools for (i) mapping and (ii) assembly. Designed for long, noisy rea...2227 days ago
Comment on "Software packages for next gen sequence analysis"
MaGuS: a tool for quality assessment and scaffolding of genome assemblies with Whole Genome Profiling™ Data MaGuS (Map-GUided Scaffolding) is a scaffolder and a re...2960 days ago
3061 days ago
Comment on "Software packages for next gen sequence analysis"
DNAMAN uses fast alignment algorithms to assemble quickly and accurately a large number of overlapping sequences. This could be a nice tutorial for genome assemblies with overlapping sequences http://www.lynnon.com/pc/assembly.html3292 days ago
Comment on "Software packages for next gen sequence analysis"
Genome assemblies tool and related papers at http://www.plosone.org/browse/Sequence+assembly+tools?startPage=53&filterAuthors=&filterSubjectsDisjunction=&filt...3295 days ago