Biological Sequence handling with Perl !
package Sequence::Generic; # File: Sequence/Generic.pm use strict; use Carp; use overload '""' => '...self->seq : $self->seq . $to_append); } 1; Back to Article...2173 days ago
2115 days ago
Installing Porechop on Ubuntu !
➜ Tools git:(master) ✗ git clone https://github.com/rrwick/Porechop.git Clo...nning install_egg_info running egg_info creating porechop.egg-info writing porechop.egg-info/PKG-INFO writing dependency_links to porechop....2098 days ago
Bash script to alignment of short reads against reference genome !
bwa mem -t 40 -R '@RG\tID:K12\tSM:K12' \ E.coli_K12_MG1655.fa SRR1770413_1.fastq....#sorting the BAM file: sambamba sort SRR1770413.raw.bam --- sort the BAM file, writing it to .sorted.bam. #marking...1551 days ago
Install and set up i-adhore for synteny and wgd analysis ! -- step by step --
#Need to download i-adhore-3.0.01.tar.gz from https://wdi...01/src/SvgWriter.cpp:24:8: warning: ‘%x’ directive writing 1 byte into a region of size...01/src/SvgWriter.cpp:24:8: warning: ‘%x’ directive writing 1 byte into a region of size...1195 days ago
1155 days ago
Commandline for paired end reads simulation with BBMap !
(JitMetaENV) ➜ mixedSample git:(main) ✗ /home/urbe/Tools/...th=250, mininsert=400, maxinsert=600, gaussian] Writing reference. Executing dna.Fas...thread=false, genscaffoldinfo=true, retain, waitforwriting=false, gz=true, maxlen=536670...982 days ago
Tadpole is 250x faster than SPADes assembler !
lege@jit-Lenovo-ideapad-320-15ISK:~/Downloads/MyTools/Vir$ tadpole.sh Written by B...with at least this depth. showstats=t Print assembly statistics after writing contigs. Prefiltering para...968 days ago