NovoGraph: building whole genome graphs from long-read-based de novo assemblies
NovoGraph: building whole genome graphs from long-read-based de novo assemblies An algorithmically novel approach to construct a genome graph representation of long-read-based de novo sequence assemblies. We then provide a proof of principle by creating a genome graph of seven ethnical...Tags: NovoGraph, building, whole, genome, graphs, long-read-based, de novo, assemblies
1996 days ago
Flye: Fast and accurate de novo assembler for single molecule sequencing reads
Flye is a de novo assembler for single molecule sequencing reads, such as those produced by PacBio and Oxford Nanopore Technologies. It is designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. The package represents a complete pipeline: it takes...Tags: Flye, Fast, accurate, de novo, assembler, single, molecule, sequencing, reads, ngs, assembly
1858 days ago
Flye: Fast and accurate de novo assembler for single molecule sequencing reads
Flye is a de novo assembler for single molecule sequencing reads, such as those produced by PacBio and Oxford Nanopore Technologies. It is designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. The package represents a complete pipeline: it takes...Tags: Fast, accurate, de novo, assembler, single, molecule, sequencing, reads
1764 days ago
The Extensive de novo TE Annotator (EDTA)
The EDTA package was designed to filter out false discoveries in raw TE candidates and generate a high-quality non-redundant TE library for whole-genome TE annotations. Selection of initial search programs were based on benckmarkings on the annotation performance using a manually curated TE libra...Tags: Extensive, de novo, TE, Annotator, EDTA
1731 days ago
Tags: Integrative, Meta-Assembly, Pipeline, IMAP, Chromosome-level, genome, assembler, multiple, de novo, assemblies
1708 days ago
NextDenovo: string graph-based de novo assembler for TGS long reads
NextDenovo is a string graph-based de novo assembler for TGS long reads. It uses a "correct-then-assemble" strategy similar to canu, but requires significantly less computing resources and storages. After assembly, the per-base error rate is about 97-98%, to further improve single base ...Tags: NextDenovo, NextOmics, string, graph-based, de novo, assembler, TGS, LongReads, assembly, long, reads
1581 days ago
3D de novo assembly (3D DNA) pipeline
For a detailed description of the pipeline and how it integrates with other tools designed by the Aiden Lab see Genome Assembly Cookbook on http://aidenlab.org/assembly. For the original version of the pipeline and to reproduce the Hs2-HiC and the AaegL4 genomes reported in (...Tags: 3D, de novo, assembly, 3D, DNA, pipeline
1552 days ago
Supernova: generates phased, whole-genome de novo assemblies from a Chromium-prepared library.
Supernova generates phased, whole-genome de novo assemblies from a Chromium-prepared library. Please see Achieving Success with De Novo Assembly and System Requirements before creating your Chromium libraries for assembly. Supernova should be run using 38-56x ...Tags: Supernova, phased, whole-genome, de novo, assembly, Chromium-prepared, library
1434 days ago
RNA-Bloom: a fast and memory-efficient de novo transcript sequence assembler
RNA-Bloom is a fast and memory-efficient de novo transcript sequence assembler. It is designed for the following sequencing data types: single-end/paired-end bulk RNA-seq (strand-specific/agnostic) paired-end single-cell RNA-seq (strand-specific/agnostic) nanopore RNA-seq (PCR...Tags: RNA-Bloom, fast, memory-efficient, de novo, transcript, sequence, assembler
1395 days ago
Meraculous: De Novo Genome Assembly with Short Paired-End Reads
We describe a new algorithm, meraculous, for whole genome assembly of deep paired-end short reads, and apply it to the assembly of a dataset of paired 75-bp Illumina reads derived from the 15.4 megabase genome of the haploid yeast Pichia stipitis. More than 95% of the genome is recovered, wi...Tags: Meraculous, De Novo, Genome, Assembly, Short, Paired-End, Reads
2370 days ago