Installing Porechop on Ubuntu !
.../home/urbe/anaconda3/bin ➜ Porechop git:(master) porechop -h usage: porechop -i INPUT [-o OUTPUT] [--format {auto,fasta,fastq,fasta.gz,fastq.gz}] [-v VERBOSITY]...2103 days ago
2096 days ago
2072 days ago
2031 days ago
1831 days ago
1722 days ago
Bash script to download SRA file !
...se the sratoolkit to directly pull the sequence data (in paired FASTQ format) from the archive. fastq-dump is in the SRA toolkit. I...irectly downloading data from a particular sequencing run ID fastq-dump --split-files SRR1770413...1556 days ago
Bash script to alignment of short reads against reference genome !
...' \ E.coli_K12_MG1655.fa SRR1770413_1.fastq.gz SRR1770413_2.fastq.gz \ | samtools view -b...2 and the sample name K12" #reference and FASTQs E.coli_K12_MG1655.fa SRR1770...4' \ E.coli_K12_MG1655.fa SRR341549_1.fastq.gz SRR341549_2.fastq.gz \...1556 days ago
To convert just one specific read group to fastq
...SRR data for each cat selected.txt | parallel fastq-dump -O reads -X 1000 --split...h SAMPLE. cat selected.txt | parallel "picard FastqToSam F1=reads/{}_1.fastq F2=r...ls view -r GROUP-SRR1972919 all.bam | samtools fastq -t -1 all_SRR1972919_1.fq -2...1539 days ago
1399 days ago