Merqury: reference-free quality and phasing assessment for genome assemblies
Often, genome assembly projects have illumina whole genome sequencing reads available for the assembled individual. The k-mer spectrum of this read set can be used for independently evaluating assembly quality without the need of a high quality reference. Merqury provides a set of tools for this ...Tags: Merqury, reference-free, quality, phasing, assessment, genome, assemblies
1422 days ago
Tags: Genobuntu, software, package, packages, oriented, NGS, assembly, genome
1965 days ago
How to estimate the heterozygosity ?
I am working on a new genome, and wondering about prediction methods for heterozygosity in the genome. Any tools suggestions and helps are welcome.Tags: heterozygosity, estimate, genome, reads
1965 days ago
genoPlotR - plot gene and genome maps project!
genoPlotR is a R package to produce reproducible, publication-grade graphics of gene and genome maps. It allows the user to read from usual format such as protein table files and blast results, as well as home-made tabular files. Features Linear representation of several segments of DNA Comp...Tags: genoPlotR, plot, gene, genome, maps, project, R
1964 days ago
Purge Haplotigs: Pipeline to help with curating heterozygous diploid genome assemblies
Some parts of a genome may have a very high degree of heterozygosity. This causes contigs for both haplotypes of that part of the genome to be assembled as separate primary contigs, rather than as a contig and an associated haplotig. This can be an issue for downstream analysis whether you're wor...Tags: Purge, Haplotigs, Pipeline, curate, heterozygous, diploid, genome, assemblies
1959 days ago
ARCS: scaffolding genome drafts with linked reads
ARCS requires two input files: Draft assembly fasta file Interleaved linked reads file (Barcode sequence expected in the BX tag of the read header or in the form "@readname_barcode" ; Run Long Ranger basic on raw chromium reads to produce this interleaved file)Tags: ARCS, scaffolding, genome, drafts, linked, reads
1959 days ago
Versatile genome assembly evaluation with QUAST-LG
QUAST-LG is an extension of QUAST intended for evaluating large-scale genome assemblies (up to mammalian-size). QUAST-LG is included in the QUAST package starting from version 5.0.0 (download the latest release). Run QUAST as usual and do not forget to add ‐‐large ...Tags: Versatile, genome, assembly, evaluation, QUAST-LG
1954 days ago
Hawkeye: an interactive visual analytics tool for genome assemblies
Genome sequencing remains an inexact science, and genome sequences can contain significant errors if they are not carefully examined. Hawkeye is our new visual analytics tool for genome assemblies, designed to aid in identifying and correcting assembly errors. Users can analyze all levels of an a...Tags: Hawkeye, interactive, visual, analytics, tool, genome, assemblies, visualization
1944 days ago