2732 days ago
ONT assembly and Illumina polishing pipeline
...wing steps: Assembly of nanopore reads using Canu. Polish canu contigs using racon (optional). Map a paired-end Illumina dataset onto the contigs obtained in the...2348 days ago
miniasm: very fast OLC-based de novo assembler for noisy long reads
...ested on a dozen of PacBio and Oxford Nanopore (ONT) bacterial data sets. Includi...bles 9 out of 12 PacBio datasets and 3 out of 4 ONT datasets into a single contig...erial genomes, it is possible to generate long contigs from raw PacBio or ONT rea...2344 days ago
2329 days ago
NanoSim: nanopore sequence read simulator based on statistical characterization.
NanoSim, a fast and scalable read simulator that captures the technology-specific features of ONT data and allows for adjustments upon improvement of nanopore sequencing technology....2323 days ago
Jabba: Hybrid Error Correction for Long Sequencing Reads
...is a hybrid error correction tool to correct third generation (PacBio / ONT) sequencing data, using secon...rections of the long reads, and additionally a file in the input format containing uncorrected reads. htt...1975 days ago
minimap2: A versatile pairwise aligner for genomic and spliced nucleotide sequences
...o.fq.gz > aln.sam # PacBio genomic reads ./minimap2 -ax map-ont ref.fa ont.fq.gz > aln.sam #...ads.fa > overlaps.paf # PacBio read overlap ./minimap2 -x ava-ont reads.fa reads.fa > overla...2139 days ago
Wtdbg2: a de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore
...for long noisy reads produced by PacBio or Oxford Nanopore Technologies (ONT). It assembles raw reads with...tens of times faster than CANU and FALCONwhile producing contigs of comparable base accurac...2018 days ago
wtdbg2: A fuzzy Bruijn graph approach to long noisy reads assembly
Wtdbg2 is a de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore Technologies (ONT). It assembles raw reads without error correction and t...1910 days ago
Flye: Fast and accurate de novo assembler for single molecule sequencing reads
...rom small bacterial projects to large mammalian-scale assemblies. The package represents a complete pipeline: it takes raw PB / ONT reads as input and outputs polished contigs. Flye also includes a spec...1853 days ago