List of tools frequently used while genome assembly
...map2 (v. 2.0rc1-r232) minimap (v. 0.2-r124-dirty) bwa (v. 0.7.12-r1039) I have used the following polishing tools Racon (v. not available) Pilo...1944 days ago
Unicycler: Hybrid assembly pipeline for bacterial genomes
...here it functions as a SPAdes-optimiser. It can also assembly long-read-only sets (PacBio or Nanopore) where it runs a miniasm+Racon pipeline. For the best p...2382 days ago
ONT assembly and Illumina polishing pipeline
This pipeline performs the following steps: Assembly of nanopore reads using Canu. Polish canu contigs using racon (optional). Map a paired-end Illumina dataset o...2369 days ago
My commonly used commands in Bioinformatics
...se MUMmer to extract the specific changes that Racon makes, so I can evaluate them...map -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 long_reads.fastq.gz - a...ssembly.fasta nucmer -p nucmer assembly.fasta racon_assembly.fasta show-snps -C...2124 days ago
Minipolish: A tool for Racon polishing of miniasm assemblies
Miniasm is a great long-read assembly tool: straight-forward, effective and very fast. However, it does not include a polishing step, so its assemblies have a high error rate – they are essentially made of stitched-together pieces of long reads. Racon is a great polishing tool th...Tags: Minipolish, tool, Racon, polishing, miniasm, assemblies
1629 days ago
Comment on "Long read assembly workshop !"
Long reads base bacterial genome assembly https://inf-biox121.readthedocs.io/en/2016/Assembly/practicals/07_Assembly_using_minasm+racon.html2053 days ago
Comment on "Run miniasm assembler on nanopore reads !"
...the PAF file) created in the fist step. These mappings are required for Racon assembly (Step 5). minimap2...ibio_oly.paf > 20170918_minimap2_mapping_fasta_oly_pacbio.paf 5 Run racon The output file is the FASTA...2181 days ago