Installing Porechop on Ubuntu !
..._threshold MIDDLE_THRESHOLD] [--extra_middle_trim_good_side EXTRA_MIDDLE_TRIM_GOOD_SIDE] [--e...(default: 85.0) --extra_middle_trim_good_side EXTRA_MIDDLE_TRIM_GOOD...2103 days ago
Install Python locally on shared Linux server !
...ll use the default python of #the system. We are going to update the environment...source ~/.bashrc_profile #You might need to logout and login again for the env...ion by the command source ~/.bashrc_profile or logout and login. Then, check if p...2095 days ago
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Generate simulated polyploid genome !
...bar -point 4 -count 16558 toy.fasta > toyheterozygous3percent.fasta #Cat both...toy.fasta toymutated3percent.fasta > toyheterozygous3percent.fasta #generated...ired-end reads sim_reads --depth 50 toyheterozygous3percent.fasta > toyheterozy...2050 days ago
Perl script to reverse complement a DNA sequence !
...$revcom =~ s/G/C/g; $revcom =~ s/C/G/g; print "Here is the reverse complement DNA: WRONG:\n\n"; print "$revcom\n"; print "\nThat was a bad algorithm, and the reverse complem...2040 days ago
Installing pb-assembly on Linux !
...libxml2/2.9.0-goolf-1.4.10 binutils/2.28-GCCc...18-GCCcore-6.4.0 bzip2/1.0.6-goalf-1.1.0-no-OFED...3.3-GCCcore-6.4.0 FFTW/3.3.4-gompi-2016b...ScaLAPACK/1.8.0-gompi-1.1.0-no-OFED-ATLAS-3.8.4-...2031 days ago
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Bash script to download SRA file !
...we get two files, one for the first and second mate in each pair. We'll use them in this format when aligning. wget ftp://ftp-trace.ncbi.nlm.nih.gov/sra/sra instant/reads/ByRun/...1556 days ago