Mapping with BWA-mem or BWA-sampe in one go with python script !
BAM files and mapping BESST requires sorted and indexed BAM files as input. Any read aligner + samtools can be used to obtain such files. Read pairs needs to be aligned in paired re...2138 days ago
2142 days ago
2141 days ago
Installing Porechop on Ubuntu !
...adapters from read ends --end_size END_SIZE The number of base pairs at each end of the read...plit read pieces smaller than this many base pairs will not be outputted (defaul...2120 days ago
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Tadpole is 250x faster than SPADes assembler !
...reads) and the outermost kmers cannot be extended. Pairs are removed only if both read...ove reads containing kmers at or below this depth. Pairs are removed if either read fa...990 days ago
Script to rapid genome clustering based on pairwise ANI
...slen' -max_target_seqs 10000 -perc_identity 90 -o -num_threads 32 Next, calculate pairwise ANI by combining local alignments between sequence pairs: anicalc.py -i -o Finall...650 days ago