Liftoff: An accurate GFF3/GTF lift over pipeline
Liftoff is a tool that accurately maps annotations in GFF or GTF between assemblies of the same, or closely-related species. Unlike current coordinate lift-over tools which require a pre-generated “chain” file as input, Liftoff is a standalone tool that takes two genome assemblies and...Tags: Liftoff, accurate, GFF3, GTF, lift, pipeline
1230 days ago
CSA: A high-throughput chromosome-scale assembly pipeline for vertebrate genomes
The pipeline can use information from scaffolded assemblies (for example from HiC or 10X Genomics), or even from diverged (~65-100 Mya) reference genomes for ordering the contigs and thus support the assembly process. This typically results in improved contig N50 when compared to current state of...Tags: CSA, high-throughput, chromosome-scale, assembly, pipeline, vertebrate, genomes
1149 days ago
AlignGraph2: similar genome-assisted reassembly pipeline for PacBio long reads
AlignGraph2 is the second version of AlignGraph for PacBio long reads. It extends and refines contigs assembled from the long reads with a published genome similar to the sequencing genome. More at https://academic.oup.com/bib/advance-article-abstract/doi/10.1093/bib/bbab022/61467...Tags: AlignGraph2, genome-assisted, reassembly, pipeline, PacBio, long, reads
1145 days ago
Tags: MCscan, pipeline, synteny, plant, annotation, comparative genomics, jcvi
1090 days ago
Tags: judi, pipeline, wokflow, doit, snakemake
970 days ago
Tags: LncPipe, Nextflow, pipeline, comprehensive, analyses, long, non-coding, RNAs, RNA-seq, datasets
959 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignment (Bowtie 2) Sort and index alignments (SAMtools) Primer sequence removal (iVar; amplicon data only) Duplicate read marking (p...Tags: assembly, pipeline, genome, illumina
874 days ago
MitoHiFi: a python pipeline for mitochondrial genome assembly from PacBio high fidelity reads
MitoHiFi v3.2 is a python pipeline distributed under MIT License ! MitoHiFi was first developed to assemble the mitogenomes for a wide range of species in the Darwin Tree of Life Project (DToL) https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-023-05385-yTags: MitoHiFi, python, pipeline, mitochondrial, genome, assembly, PacBio, fidelity, reads
240 days ago
PhyloHerb: A high‐throughput phylogenomic pipeline for processing genome skimming data
Phylogenomic Analysis Pipeline for Herbarium Specimens What is PhyloHerb: PhyloHerb is a wrapper program to process genome skimming data collected from plant materials. The outcomes include the plastid genome (plastome) assemblies, mitochondrial genome assemblies, nuclear ribosoma...Tags: PhyloHerb, high‐throughput, phylogenomic, pipeline, processing, genome, skimming, data
240 days ago
pipesnake: bioinformatics best-practice analysis pipeline for phylogenomic reconstruction
ausarg/pipesnake is a bioinformatics best-practice analysis pipeline for phylogenomic reconstruction starting from short-read 'second-generation' sequencing data. The pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portab...Tags: pipesnake, bioinformatics, best-practice, analysis, pipeline, phylogenomic, reconstruction
71 days ago