Create genome scaffolding with Perl
#!/usr/bin/perl use warnings; use strict; use English; use Pod::...prefix=s'); # process remaining command line arguments (hopefully onl...-message => "Error: Unknown command-line option or "....t sequence is assumed to be forward strand...2299 days ago
Biological Sequence handling with Perl !
package Sequence::Generic; # File: Sequence/Generic.pm use str...' residues)'; } # Concatenate two sequences together and return the result sub conc...: $self->seq . $to_append); } 1; Back to Article...2172 days ago
2114 days ago
Installing Porechop on Ubuntu !
➜ Tools git:(master) ✗ git clone https://github.com/rrwick/Porechop.gi...info creating porechop.egg-info writing porechop.egg-info/PKG-INFO writing dependency_links to porec...ads, trimming them from the ends and splitting reads with internal...are saved to a file and stde...2097 days ago
Bash script to alignment of short reads against reference genome !
bwa mem -t 40 -R '@RG\tID:K12\tSM:K12' \...says "align using so many threads" and also "give the reads the read group K12 and the sample name K12" #refere...ce of the file name indicates this) and converts to BAM. #sorting th...0413.raw.bam --- sort the BAM file, writing...1550 days ago
Installing ggplot2 and its dependencies on Ubuntu !
jit@jit-HP-Pro-3335-MT:~/Downloads/MitoHunter/minidot/bin$ sudo...‘glue’ successfully unpacked and MD5 sums checked ** libs gc...* R ** inst ** byte-compile and prepare package for lazy load..._FORTIFY_SOURCE=2 -g -c stri_random.cpp -o stri_random.o g++ -...ad.o stri_prepare_arg.o stri_random.o...1541 days ago
Install and set up i-adhore for synteny and wgd analysis ! -- step by step --
#Need to download i-adhore-3.0.01.tar.gz from https://wdiceryfd4rj...rc/bmp/CMakeFiles/bmp.dir/pnghandling.cp...24:8: warning: ‘%x’ directive writing 1 byte into a region of size between 0 and 6 [-Wformat-overflow=] stri...sprintf_chk’ output between 8 and 26 bytes into a destination o...1194 days ago
1154 days ago
Commandline for paired end reads simulation with BBMap !
(JitMetaENV) ➜ mixedSample git:(main) ✗ /home/urbe/Tools/bbmap/randomreads.../Tools/bbmap/current/ align2.RandomReads3 build=1 ref=mixed.fa...0 gaussian Executing align2.RandomReads3 [build=1, ref=mixed.f..., maxinsert=600, gaussian] Writing reference. Executing dna...981 days ago
Tadpole is 250x faster than SPADes assembler !
lege@jit-Lenovo-ideapad-320-15ISK:~/Down...ut= mode=correct Extension and correction may be done simult...int assembly statistics after writing contigs. Prefiltering...faster and more memory-efficient. A flo...xtend sequences to be longer, and optionally...967 days ago