Comment on "A quick guide to Phred scaling"
...e base call. Quality score encoding: The Phred quality scores are typically encoded in the quality score field of the FASTQ file format, which is a common format used for storing sequencing r...406 days ago
Comment on "Short-read assembly using Spades !"
...the directory where the assembled genome will be saved, "forward_reads.fastq" and "reverse_reads.fastq" are the forward and reverse reads in FASTQ format. Evaluation of assembly:...406 days ago
Comment on "Short-read assembly using Spades !"
...systems. Prepare the input data: The input data should be in FASTQ format, which contains the sequencin...gth or enabling error correction. The SPAdes manual provides detailed information on how to use these option...412 days ago
Comment on "Tools for Sequence translation !"
...ioPython: This is a Python library that provides several tools for bioinformatics analysis, including sequen...l depends on the specific requirements of the user, including the input format, the genetic code used, and t...412 days ago
Comment on "Basic command-line to run BLAST"
...Prepare the query sequence: The query sequence should be in a FASTA format and can be entered directly i...mplete, the results can be viewed and downloaded. The results include information about the sequences that m...414 days ago
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Comment on "URMAP, an ultra-fast read mapper"
...A file for the reference is not needed. Reads must be in FASTQ format or gzip-compressed FASTQ format. It is generally faster to us...index filename is given by the -ufi option. A filename for SAM format output is specified by -samou...1274 days ago
Comment on "Sibelia: A comparative genomics tool"
...ailed depiction of the output format. The "far" parameters set ca...s block are listed in tabular format. Each row of a table depicts...uence has header in following format: >Seq="<header>"...rameters has following simple format: M K1 D1 K2 D2 .....1276 days ago
Comment on "Mugsy: multiple whole genome alignment tool"
...the FASTA files should not contain ':' or '-' to avoid parsing problems. View or parse MAF output The output of Mugsy is Multiple Alignment Format (MAF).One option for browsing...1276 days ago
Comment on "RGFA: powerful and convenient handling of assembly graphs"
The Graphical Fragment Assembly (GFA) is a proposed format which allow to describe the product of sequence assembly. This gem implements the proposed specifications for the GFA format described under https://github.com/GFA-spec/GFA-spec/blob/master/GFA-spec.md as close as possible.1558 days ago