Results for "ONT"

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  • ONT assembly and Illumina polishing pipeline

    This pipeline performs the following steps: Assembly of nanopore reads using Canu. Polish canu contigs using racon (optional). Map a paired-end Illumina dataset onto the contigs obtained in the previous steps using BWA mem. Perform correction of contigs using p...

    Tags: ONT, assembly, Illumina, polishing, pipeline

    25 days ago

  • SPAdes hybrid genome assembly

    When you have both Illumina and Nanopore data, then SPAdes remains a good option for hybrid assembly - SPAdes was used to produce the B fragilis assembly by Mick Watson’s group. Again, running spades.py will show you the options: spades.py This produces: SPAdes genome ...

    Tags: SPAdes, hybrid, genome, assembly, ONT, Nanopore

    21 days ago

  • MashMap: a fast and approximate software for mapping long reads (PacBio/ONT) or assembly to reference genome(s)

    MashMap is a fast and approximate software for mapping long reads (PacBio/ONT) or assembly to reference genome(s). It maps a query sequence against a reference region if and only if its estimated alignment identity is above a specified threshold. It does not compute the alignments explicitly, but...

    Tags: MashMap, fast, approximate, software, mapping, long, reads, PacBio, ONT, assembly, reference, genome(s)

    5 days ago