I recommend to have a look at this BOL
For De-novo Genome Assembly -- Canu. Flye and MARVEL sound promising.Alignment -- minimap2 and/or LAST, depending on the application.
LAST is particularly good...
MARVEL consists of a set of tools that facilitate the overlapping, patching, correction and assembly of noisy (not so noisy ones as well) long reads.
The assembly process can be summarized as follows:
overlap
patch reads
overlap...
Flye is a de novo assembler for long and noisy reads, such as those produced by PacBio and Oxford Nanopore Technologies. The algorithm uses an A-Bruijn graph to find the overlaps between reads and does not require them to be error-corrected. After...
Following methods can help to access the assembly correctness:
Remove the contaminated reads, map the it. If many reads didn't aligned you probably miss some regions in your assembly.
Use a reference genome, and if there are more than 2/MY...
ALPACA requires Celera Assembler 8.3 or later. It is recommended to build Celera Assembler from source. (Why? The pre-built binaries CA_8.3rc1 and CA8.3rc2 will work for any large data set.
Detail paper...