BOL

I am a computer science studentm and it might be a stupid question, but am curious to know about it. Please help be to understand this properly.

Hit:12 http://download.opensuse.org/repositories/isv:/ownCloud:/desktop/Ubuntu_16.10 Packages
Fetched 1.496 B in 0s (3.442 B/s)
Reading package lists... Done
W: GPG error: http://download.opensuse.org/repositories/isv:/ownCloud:/desktop/Ubuntu_16.10 Release: The following signatures couldn't be verified because the public key is not available: NO_PUBKEY 4ABE1AC7557BEFF9
W: The repository 'http://download.opensuse.org/repositories/isv:/ownCloud:/desktop/Ubuntu_16.10 Release' is not signed.
N: Data from such a repository can't be authenticated and is therefore potentially dangerous to use.
N: See apt-secure(8) manpage for repository creation and user configuration details.

I found it hard to make a standalone application using Python. Can you pleqse suggest me a easy zay out?

I run MIRA on my dataset and got the following error. I have /tmp directory mounted on non-NFS protocol. I wanted to use it for temporary location. How to do that? Sorry, M new to MIRA.

WARNING WARNING WARNING!

It looks like the directory MIRA uses for temporary files
/home/user/MIRA/mira_4.0.2_linux-gnu_x86_64_static//Test_Asbly_d_tmp_MJYclL
is on a NFS (Network File System) mount. This will slow down MIRA *considerably*
... by about a factor of 10!

If you don't want that, you have three possibilities:

1) RECOMMENDED! Use -DI:trt to redirect the tmp directory somewhere else on a
local disk or even SSD.
2) ALSO POSSIBLE: put the whole project somewhere else on your file system.
3) ABSOLUTELY NOT RECOMMENDED AT ALL: use "-NW:cnfs=warn" to tell MIRA not
to stop when it finds the tmp directory on NFS.

If you do not know what NFS is and which directory to use in "-DI:trt", ask
your local system administrator to guide you.

I wanted to remove the duplicates from fastq file. I planned to use fastuniq but it does not have good tutorial. Can you please tell me how to use it?

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052249

Looking for files named in data ...Pushing back filename: "clean_a2017.R1.fq"
Could not determine filetype from data 'clean_Avaga2017.R1.fq' ("clean_a2017.R1.fq")
Pushing back filename: "clean_a2017.R2.fq"
Could not determine filetype from data 'clean_a2017.R2.fq' ("clean_a2017.R2.fq")

Fatal error (may be due to problems of the input data or parameters):

********************************************************************************
* Some 'data' entries named in the manifest file could not be verified, see *
* the log above. *
* Maybe some files are missing, not readable or there is a typo in the *
* manifest file? *
********************************************************************************

I usually report and estimate the genome size by looking into file size of the contigs. Is this right way? 

In school the teachers refer to both terms as one, but according to what I have read they are not the same and don't use the same methologies so I am a bit confused.