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Abhi on LoReTTA, a user-friendly tool for assembling viral genomes from PacBio sequence data (639 days ago): Detail paper here https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111061/- BioStar on Perl Module Installation (1096 days ago): To install BioPerl on a macOS system, you can follow these steps: Open the...
- Abhi on CovCal: Coverage / Read Count Calculator (1257 days ago): Sequencing coverage is defined as the average number of reads that covers...
- Neel on CheckM:Assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes (1309 days ago): MOre at https://bitbucket.org/berkeleylab/checkv/src/master/
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Oxford Nanopore Sequencing, Hybrid Error Correction, and de novo Assembly of a Eukaryotic Genome
By Jit 3023 days agoschatzlab.cshl.edu - Monitoring the progress of DNA molecules through a membrane pore has been postulated as a method for sequencing DNA for several decades. Recently, a nanopore-based sequencing instrument, the Oxford Nanopore MinION, has become available that we used...simNGS and simLibrary – Software for Simulating Next-Gen Sequencing Data
By Jit 3024 days agowww.ebi.ac.uk - simNGS is software for simulating observations from Illumina sequencing machines using the statistical models behind the AYB base-calling software. By default, observations only incorporate noise due to sequencing and do not incorporate effects from...DRAWID: user-friendly Java software for chromosome measurements and idiogram drawing
By Robert M Willioms 3024 days agocompcytogen.pensoft.net - "DRAWID has number of advantages including a user-friendly interactive interface, possibility for simultaneous chromosome and FISH/GISH/banding signal measurement and idiogram drawing as well as number of useful functions facilitating the procedure...INC-Seq: accurate single molecule reads using nanopore sequencing
By Jit 3025 days agogithub.com - INC-Seq reads enabled accurate species-level classification, identification of species at 0.1 % abundance and robust quantification of relative abundances, providing a cheap and effective approach for pathogen detection and microbiome profiling...Opera: An optimal genome scaffolding program
By Jit 3025 days agosourceforge.net - Opera (Optimal Paired-End Read Assembler) is a sequence assembly program (http://en.wikipedia.org/wiki/Sequence_assembly ). It uses information from paired-end or long reads to optimally order and orient contigs assembled from...RITA: Rapid identification of high-confidence taxonomic assignments for metagenomic data
By Jit 3025 days agokiwi.cs.dal.ca - RITA is a standalone software package and Web server for taxonomic assignment of metagenomic sequence reads. By combining homology predictions from BLAST or UBLAST with compositional classifications from a Naive Bayes classifier, RITA is able to...miniasm: very fast OLC-based de novo assembler for noisy long reads
By Jit 3025 days agogithub.com - Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream...coursera genome assembly tutorial
By Jit 3027 days agogithub.com - Solutions to Coursera Genome Sequencing (Bioinformatics II)SIMA C++ Implementation: Simultaneous Multiple Alignment of LC/MS Peak Lists
By Neel 3028 days agohciweb.iwr.uni-heidelberg.de - This is the c++ implementation for SIMA - Simultaneous Multiple Alignment of LC/MS Peak Lists. The package contains C++ source code as well as two binary files. The latter were tested under various operating systems, including Windows XP SP3 32bit,...IONiseR: tools for the quality assessment of data produced by Oxford Nanopore’s MinION sequencer
By Jit 3029 days agowww.bioconductor.org - This package is intended to provide tools for the quality assessment of data produced by Oxford Nanopore’s MinION sequencer. It includes a functions to generate a number plots for examining the statistics that we think will be useful for this...
