In the world of transcriptomics, quantifying gene and transcript expression accurately and efficiently is crucial. With the explosion of RNA-Seq data, researchers have turned to fast, alignment-free tools that streamline the quantification process...
R.A Jobs opportunity in Jawaharlal Nehru University (JNU) on purely temporary basis
Project Title : "Structural and functional characterization of serine biosynthetic pathway enzymes from Entamoeba histolytica"
No. of Post :...
hibberdlab.com - Transrate is software for de-novo transcriptome assembly quality analysis. It examines your assembly in detail and compares it to experimental evidence such as the sequencing reads, reporting quality scores for contigs and assemblies. This...
Prioritizing missense variants for further experimental investigation is a key challenge in current sequencing studies for exploring complex and Mendelian diseases. A large number of in silico tools have been employed for the task of...
github.com - VcfR is an R package intended to allow easy manipulation and visualization of variant call format (VCF) data. Functions are provided to rapidly read from and write to VCF files. Once VCF data is read into R a parser function extracts matrices from...
The Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. in Dortmund is looking for a Post-doctoral position / Scientist (m/f/d) in Bioinformatics
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the sequenced reads can be mapped to the organism’s genes to assess how differently the genes are expressed under the experimental circumstances as opposed to the control scenario. This is known as differential expression (DE) analysis
kallisto
Transcript abundance quantification from RNA-seq data (uses pseudoalignment for rapid determination of read compatibility with targets)
Software (C++)
https://pachterlab.github.io/kallisto/
Sailfish
Estimation of isoform abundances...
github.com - HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (both DNA and RNA) to a population of human genomes (as well as to a single reference genome). Based on an extension of BWT for...