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Smudgeplot: Inference of ploidy and heterozygosity structure using whole genome sequencing data
By Neel 1001 days agogithub.com - This tool extracts heterozygous kmer pairs from kmer count databases and performs gymnastics with them. We are able to disentangle genome structure by comparing the sum of kmer pair coverages (CovA + CovB) to their relative coverage (CovB / (CovA +...GFinisher: a new strategy to refine and finish bacterial genome assemblies
By Jit 2311 days agogfinisher.sourceforge.net - GFinisher is an application tools for refinement and finalization of prokaryotic genomes assemblies using the bias of GC Skew to identify assembly errors and organizes the contigs/scaffolds with genomes references. java -Xms2G -Xmx4G -jar...coursera genome assembly tutorial
By Jit 2554 days agogithub.com - Solutions to Coursera Genome Sequencing (Bioinformatics II)Genobuntu: A software package containing more than 70 software and packages oriented towards NGS...
By BioStar 2173 days agosourceforge.net - Genobuntu is a software package containing more than 70 software and packages oriented towards NGS. In its current version, Genobuntu supports pre assembly tools, genome assemblers as well as post assembly tools. Commonly used biological...Genome assembly forensics: finding the elusive mis-assembly
By Jit 2127 days agoamos.sourceforge.net - We present the first collection of tools aimed at automated genome assembly validation. This work formalizes several mechanisms for detecting mis-assemblies, and describes their implementation in our automated validation pipeline,...StringTie Transcript assembly and quantification for RNA-Seq
By Jit 1627 days agoccb.jhu.edu - StringTie is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length...BlobToolKit: A toolkit for genome assembly QC
By Jit 1736 days agoblobtoolkit.genomehubs.org - Filtering raw genomic datasets is essential to avoid chimeric assemblies and to increase the validity of sequence-based biological inference. BlobToolKit extends the BlobTools1/Blobology2 approach to simplify interactive and reproducible...Supernova: generates phased, whole-genome de novo assemblies from a Chromium-prepared library.
By Jit 1636 days agosupport.10xgenomics.com - Supernova generates phased, whole-genome de novo assemblies from a Chromium-prepared library. Please see Achieving Success with De Novo Assembly and System Requirements before creating your Chromium libraries for...Genome assembly training tutorial at Galaxy !
By Jit 1426 days ago Comments (1)training.galaxyproject.org - In this tutorial we assemble and annotate the genome of E. coli strain C-1. This strain is routinely used in experimental evolution studies involving bacteriophages. For instance, now classic works by Holly Wichman and Jim Bull (Bull 1997, Bull...IVA: accurate de novo assembly of RNA virus genomes
By Neel 1248 days agogithub.com - IVA (Iterative Virus Assembler) designed specifically for read pairs sequenced at highly variable depth from RNA virus samples. We tested IVA on datasets from 140 sequenced samples from human immunodeficiency virus-1 or influenza-virus-infected...
