Jit
Enthusiast computational biologist with business dream !!!
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poRe: an R package for the visualization and analysis of nanopore sequencing data
By Jit 2558 days ago Comments (1)academic.oup.com - Motivation: The Oxford Nanopore MinION device represents a unique sequencing technology. As a mobile sequencing device powered by the USB port of a laptop, the MinION has huge potential applications. To enable these applications, the...nanofilt: Filtering and trimming of long read sequencing data
By Jit 2309 days ago Comments (1)github.com - Filtering on quality and/or read length, and optional trimming after passing filters.Reads from stdin, writes to stdout. Intended to be used: directly after fastq extraction prior to mapping in a stream between extraction and...npScarf: Scaffolding and Completing Assemblies in Real-time Fashion
By Jit 2743 days agogithub.com - npScarf (jsa.np.npscarf) is a program that scaffolds and completes draft genomes assemblies in real-time with Oxford Nanopore sequencing. The pipeline can run on a computing cluster as well as on a laptop computer for microbial datasets. It...LoRMA: A tool for correcting sequencing errors in long reads
By Abhimanyu Singh 2271 days agogite.lirmm.fr - An error correction method that uses long reads only. The method consists of two phases: first, we use an iterative alignment-free correction method based on de Bruijn graphs with increasing length of k-mers, and second, the corrected reads are...AlignGraph2: similar genome-assisted reassembly pipeline for PacBio long reads
By Rahul Nayak 1351 days agogithub.com - AlignGraph2 is the second version of AlignGraph for PacBio long reads. It extends and refines contigs assembled from the long reads with a published genome similar to the sequencing genome. More...Want to Know which genome assembler rule the world ?
Started by Rahul Agarwal 4123 days ago Replies (3) Last reply by Rahul Nayak 2154 days agoAssemblathon 2: evaluating de novo methods of genome...YASRA: Reference based assembler
By Abhimanyu Singh 2825 days agogithub.com - YASRA (Yet Another Short Read Assembler) performs comparative assembly of short reads using a reference genome, which can differ substantially from the genome being sequenced. Mapping reads to reference genomes makes use of LASTZ (Harris et al), a...BASE: a practical de novo assembler for large genomes using long NGS reads
By Rahul Nayak 2229 days agogithub.com - new de novo assembler called BASE. It enhances the classic seed-extension approach by indexing the reads efficiently to generate adaptive seeds that have high probability to appear uniquely in the genome. Such seeds form the basis for BASE...Tadpole: an assembler, error-corrector, and read-extender
By BioStar 1755 days agojgi.doe.gov - Tadpole is a kmer-based assembler, with additional capabilities of error-correcting and extending reads. It does not do any complicated graph analysis or scaffolding, and therefore, is not particularly good for diploid organisms. Tadpole is...Comparison of Short Read De Novo Alignment Algorithms
By Rahul Agarwal 4114 days agobiochem218.stanford.edu - Excellent article to introduce different sequencing methods along with tools for de novo assembly of sequencing reads and their relevant references. Title: Comparison of Short Read De Novo Alignment Algorithms Author: Nikhil Gopal
