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Protocol for De novo Genome Assembly using Illumina Reads
By BioStar 1409 days agoIn this protocol, we address and describe the de novo assembly method for small to medium-sized genomes.JBrowse: Embeddable genome browser built completely with JavaScript and HTML5
By Jit 2341 days agogithub.com - JBrowse is a fast, embeddable genome browser built completely with JavaScript and HTML5, with optional run-once data formatting tools written in Perl. Headline Features: Fast, smooth scrolling and zooming. Explore your genome with unparalleled...GenomeMapper: Simultaneous alignment of short reads against multiple genomes
By Jit 2376 days ago1001genomes.org - GenomeMapper is a short read mapping tool designed for accurate read alignments. It quickly aligns millions of reads either with ungapped or gapped alignments. It can be used to align against multiple genomes simulanteously or against a single...CoLoRMap: Correcting Long Reads by Mapping short reads
By Jit 2289 days agogithub.com - Second generation sequencing technologies paved the way to an exceptional increase in the number of sequenced genomes, both prokaryotic and eukaryotic. However, short reads are difficult to assemble and often lead to highly fragmented assemblies....wtdbg2: A fuzzy Bruijn graph approach to long noisy reads assembly
By BioStar 2121 days agogithub.com - Wtdbg2 is a de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore Technologies (ONT). It assembles raw reads without error correction and then builds the consensus from intermediate assembly...HapSolo: An optimization approach for removing secondary haplotigs during diploid genome assembly...
By Jit 1297 days agogithub.com - HapSolo, that identifies secondary contigs and defines a primary assembly based on multiple pairwise contig alignment metrics. HapSolo evaluates candidate primary assemblies using BUSCO scores and then distinguishes among candidate assemblies using...COPE: an accurate k-mer-based pair-end reads connection tool to facilitate genome assembly
By Jit 2546 days agoftp.genomics.org.cn - An efficient tool called Connecting Overlapped Pair-End (COPE) reads, to connect overlapping pair-end reads using k-mer frequencies. We evaluated our tool on 30× simulated pair-end reads from Arabidopsis thaliana with 1% base error. COPE...Cerulean: A hybrid assembly using high throughput short and long reads
By Rahul Nayak 2365 days agosourceforge.net - Cerulean extends contigs assembled using short read datasets like Illumina paired-end reads using long reads like PacBio RS long reads. Cerulean v0.1 has been implemented with bacterial genomes in mind. The method is fully described in...LR_Gapcloser: a tiling path-based gap closer that uses long reads to complete genome assembly
By Rahul Nayak 1656 days agogithub.com - LR_Gapcloser is a gap closing tool using long reads from studied species. The long reads could be downloaed from public read archive database (for instance, NCBI SRA database ) or be your own data. Then they are fragmented and aligned to scaffolds...HASLR: a hybrid assembler which uses both second and third generation sequencing reads
By BioStar 1666 days agogithub.com - HASLR, a hybrid assembler which uses both second and third generation sequencing reads to efficiently generate accurate genome assemblies. Our experiments show that HASLR is not only the fastest assembler but also the one with the lowest number of...
