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kallisto: a program for quantifying abundances of transcripts from bulk and single-cell RNA-Seq data
By Jit 2714 days ago Comments (1)pachterlab.github.io - kallisto is a program for quantifying abundances of transcripts from bulk and single-cell RNA-Seq data, or more generally of target sequences using high-throughput sequencing reads. It is based on the novel idea of pseudoalignment for...Modular, efficient and constant-memory single-cell RNA-seq preprocessing
By Jit 1895 days ago Comments (1)pachterlab.github.io - With kallisto | bustools you can Generate a cell x gene or cell x transcript equivalence class count matrix Perform RNA velocity and single-nuclei RNA-seq analsis Quantify data from numerous technologies such as...StringTie Transcript assembly and quantification for RNA-Seq
By Jit 2195 days agoccb.jhu.edu - StringTie is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length...QuasR: Quantification and annotation of short reads in R
By Neel 1765 days agowww.bioconductor.org - The QuasR package (short for Quantify and annotate short reads in R) integrates the functionality of several R packages (such as IRanges (Lawrence et al. 2013) and Rsamtools) and external software (e.g. bowtie, through the Rbowtie package, and...RNA-Seq De novo Assembly Using Trinity
By Surabhi Chaudhary 3734 days agogithub.com - Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Trinity combines three independent software modules:...NCBI Magic-BLAST
By Jit 2860 days agoncbi.github.io - Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome. Each alignment optimizes a composite score, taking into account simultaneously the two reads of a pair, and in case of...RNA-seq workflow: gene-level exploratory analysis and differential expression
By Jit 1708 days agomaster.bioconductor.org - Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were quantified to the reference transcripts, and prepare gene-level count...
Pathway Analysis
Last updated 4265 days ago by Rahul Agarwal Comments (2)Pathway Analysis is usually performed with aim to enrich the genes with their functional information and reveal the underlying biological mechanisms pursue by genes. Pathway Analysis is not only limited to what biological pathways a particular set...Integrative RNA and ChIP-Seq analysis of regulatory T-cells
By Strand 3966 days agoIntegrative RNA and ChIP-Seq analysis of regulatory T-cellsWebinar on Unique Molecular Identifier (UMI)-powered Ultra-sensitive Variant Calling using Strand...
By Strand 3140 days ago Comments (1)Webinar on Unique Molecular Identifier-powered Ultra-sensitive Variant Calling using Strand NGS - Case Study by Dr. Pandurang Kolekar, Bioinformatics Engineer, Strand Life Sciences Abstract: Unique Molecular Identifiers (UMIs) are short...
