Comment on "A quick guide to Phred scaling"
...used system for representing the quality scores of sequencing reads. I...uide to Phred scaling: Phred quality scores: Phred quality scores...ility of error of 1 in 1000. Quality score calculation: The Phred...or storing sequencing reads. Each quality score is represented as an AS...458 days ago
Comment on "Short-read assembly using Spades !"
...ps for short-read assembly using SPAdes: Quality control of raw reads: Before...tant to ensure that the raw reads are of high quality and do not contain sequencing...ning SPAdes, the next step is to evaluate the quality of the assembled genome. This...458 days ago
Comment on "Nextflow: Data-driven computational pipelines"
A collection of high quality Nextflow pipelines https://github.com/nf-core/1579 days ago
Comment on "Important Journals, Blogs and Forums for Bioinformaticians"
NAR: Genomics and Bioinformatics publishes high quality research into genomics and bioinformatics, including novel computational or experimental methods and novel biological findings related to genomics, proteomics, transcriptomics, and epigenetics. https://academic.oup.com/nargab1902 days ago
2142 days ago
Comment on "Running Trinity on RNA-seq !"
...and gene abundance. This is a prerequisite to many other analyses such as examining differentially expressed transcripts among your samples. Quality checking your samples and bio...2166 days ago
2180 days ago
Comment on "GraphMap - A highly sensitive and accurate mapper for long, error-prone reads"
...0.0, thredhold not applied. [1e0] -c, --mapq INT Threshold for mapping quality. If mapq < INT, read will...given fraction of the top score will be counted for ambiguity (mapping quality). Value of 0.0 counts only id...2335 days ago
2842 days ago
Comment on "Software packages for next gen sequence analysis"
MaGuS: a tool for quality assessment and scaffolding of genome assemblies with Whole Genome Profili...d Scaffolding) is a scaffolder and a reference-free evaluator of assembly quality. It uses a draft genome assem...2951 days ago