Comment on "Run miniasm assembler on nanopore reads !"
minimap2 –x ava-ont \ ../../trimming_practical/nanofilt/nanofilt_trimmed.fastq \ ../../trimming_practical/nanofilt/nanofilt_trimmed.fastq \ | gzip -1 >...838 days ago
Comment on "Extract the values using ids !"
Join could be also useful linux_prompt> join file1 file2 11002 item 6 10995 item 7 79600 item 7936 days ago
Comment on "Command line to create blast uniref database !"
wget ftp://ftp.uniprot.org/pub/databases/uniprot/uniref/uniref90/uniref90.fasta.gz gunzip -v uniref90.fasta.gz makeblastdb -in uniref90.fasta -dbtype prot -input_type fasta -out uniref90.fasta987 days ago
Comment on "Far Manager Commands and Links !"
KeyDescription F1 Yes! Read it first. BTW, FarEng.hlf is a simple text file, you can open it in any editor. ShiftF1 Create new archive...1002 days ago
Comment on "Basic docker commands !"
Attached the running docker Instead of using -i or -t options, use the attach command to connect to a running container: docker attach container_id1014 days ago
Comment on "Calling variants in non-diploid systems"
FreeBayes is widely used for calling variants in diploid systems. However, it can also be used for calling variants in pooled samples where the number of samples is no...1085 days ago
Comment on "How to install Perl modules manually, using CPAN command, and other quick ways"
# envname is the name of your (existing) conda env conda activate envname # cpanm is available on either conda-forge or # bioconda, so add conda-forge (or look up...1223 days ago
Comment on "Installing BLAT on Linux !"
Get the code. The best way to do this now for Unix users is via Git following the instructions at: http://genome.ucsc.edu/admin/git.html Or, fetch the entire source in a...1252 days ago
Comment on "KAT: a K-mer analysis toolkit to quality control NGS datasets and genome assemblies"
Using KAT again (You will need the modules: KAT/2.1.1 and gnuplot/4.6.5) – we can plot the kmer content of the assembly compared to the kmer conte...1378 days ago
Comment on "REAPR: a universal tool for genome assembly evaluation"
Reapr is a tool trying to find explicit errors in the assembly based on incongruently mapped reads. It is heavily based on too low span coverage, or reads mapping...1378 days ago