MitoZ: a toolkit for animal mitochondrial genome assembly, annotation and visualization
MitoZ, consisting of independent modules of de novo assembly, findMitoScaf (find Mitochondrial Scaffolds), annotation and visualization, that can generate mitogenome assembly together with annotation and visualization results from HTS raw reads. https://academic.oup.com/nar/article/47/11/e63/537...Tags: MitoZ, toolkit, animal, mitochondrial, genome, assembly, annotation, visualization
917 days ago
ncbi-datasets-cli -- Quickstart: command line tools !
Install and use the NCBI Datasets command line tools The NCBI Datasets datasets command line tools are datasets and dataformat . Use datasets to download biological sequence data across all domains of life from NCBI. Use dataformat to convert metadata fr...Tags: ncbi-datasets-cli, database, download, ncbi, genome, genes, datasets
911 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignment (Bowtie 2) Sort and index alignments (SAMtools) Primer sequence removal (iVar; amplicon data only) Duplicate read marking (p...Tags: assembly, pipeline, genome, illumina
908 days ago
Refseq viraal genome sequences !
List of all viruses on NCBI https://ftp.ncbi.nlm.nih.gov/refseq/release/viral/Tags: nvbi, viruses, genome, data, ftp
907 days ago
Tags: maftools, maf, format, snp, variant, genome, mutation
901 days ago
PLAR: Pipeline for lncRNA annotation from RNA-seq data
Due to several requests, we are releasing an assingment of orthologs, determined using the same methods used in Hezroni et al. (BLAST, Whole Genome Alignment (WGA), and synteny). One is comparing human GENCODE genes (from GENCODE v30) to lncRNAs from other species identified by PLAR. Available&nb...Tags: lncRNA, data, human, genome, plar
880 days ago
Tags: bwa, mapping, genome, vcf, align, genome
867 days ago
CrossMap: program for genome coordinates conversion between different assemblies
CrossMap is a program for genome coordinates conversion between different assemblies (such as hg18 (NCBI36) <=> hg19 (GRCh37)). It supports commonly used file formats including BAM, CRAM, SAM, Wiggle, BigWig, BED, GFF, GTF,&nb...Tags: CrossMap, program, genome, coordinates, conversion, different, assemblies
861 days ago
Short-read assembly using Spades !
If we only had Illumina reads, we could also assemble these using the tool Spades. You can try this here, or try it later on your own data. Get data We will use the same Illumina data as we used above: illumina_R1.fastq.gz: the Illumina forward reads illumina_R2.fastq.gz: the Illumina reve...Tags: spades, assembly, short, reads, genome, tools
856 days ago
odgi: optimized dynamic genome/graph implementation
odgi provides an efficient and succinct dynamic DNA sequence graph model, as well as a host of algorithms that allow the use of such graphs in bioinformatic analyses. Careful encoding of graph entities allows odgi to efficiently compute and transform pangenomes with mini...Tags: odgi, optimized, dynamic, genome, graph, implementation, genome, assembly, graph
854 days ago