Comment on "Short-read assembly using Spades !"
SPAdes (St. Petersburg genome assembler) is a popular tool for short-read assembly. It can assemble reads generated from Illumina, IonTorrent, PacBi...439 days ago
Comment on "Basic command-line to run BLAST"
Following steps need to followed while doing any homology search. BLAST (Basic Local Alignment Search Tool) is a widely used program for sear...441 days ago
Comment on "Run miniasm assembler on nanopore reads !"
minimap2 –x ava-ont \ ../../trimming_practical/nanofilt/nanofilt_trimmed.fastq \ ../../trimming_practical/nanofilt/nanofilt_trimmed.fastq \ | gzip -1 > ....814 days ago
Comment on "Command line to create blast uniref database !"
wget ftp://ftp.uniprot.org/pub/databases/uniprot/uniref/uniref90/uniref90.fasta.gz gunzip -v uniref90.fasta.gz makeblastdb -in uniref90.fasta -dbtype prot -input_type fasta -out uniref90.fasta964 days ago
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Comment on "URMAP, an ultra-fast read mapper"
map command Maps unpaired reads to a reference genome. The reference genome is stored as a UFI file created by the make_ufi command. The original FASTA file for t...1302 days ago
Comment on "Sibelia: A comparative genomics tool"
Basic usage =========== In this manual it is assumed that "Sibelia" is properly installed and the directory with "Sibelia.py" is in your "PATH" environment variable...1303 days ago
Comment on "Mugsy: multiple whole genome alignment tool"
Set up the environment bash source /path/to/install/mugsyenv.sh Run mugsy mugsy --directory /data/output -...1304 days ago
Comment on "KAT: a K-mer analysis toolkit to quality control NGS datasets and genome assemblies"
Using KAT again (You will need the modules: KAT/2.1.1 and gnuplot/4.6.5) – we can plot the kmer content of the assembly compared to the kmer conten...1355 days ago