413 days ago
Comment on "Short-read assembly using Spades !"
...rsburg genome assembler) is a popular tool for short-read assembly. It can assemble reads generated from Illumina, IonTorrent, PacBio, and Oxford Nanopore sequencing platforms. Here is...418 days ago
Comment on "Want to Know which genome assembler rule the world ?"
Canu Assembler is my personal favourate.Canu is a fork of the Celera Assembler, designed for high-noise single-molecule sequencing (such as the PacBio RS II/Sequelor Oxford Nanopore MinION).1948 days ago
Comment on "nanofilt: Filtering and trimming of long read sequencing data"
Nanofilt steps https://gigabaseorgigabyte.wordpress.com/2017/06/05/trimming-and-filtering-oxford-nanopore-sequencing-reads/2037 days ago
2096 days ago
Comment on "LAMSA: fast split read alignment with long approximate matches"
...rs unless overriden. [null] (Illumina Moleculo) pacbio (PacBio SMRT): -i25 -l50 -m1 -M1 -O1,1,2,2 -E1,1,1,1 -w200 -b0 -e0.30 -d0.30 ont2d (Oxford Nanopore): -i25 -l50 -m1 -M1 -O1,1,1,1...2120 days ago
Comment on "SPAdes hybrid genome assembly"
...6.2-Linux/bin/spades.py --only-assembler -t 4 -k 21,51,71 -1 SRR2627175_1.fastq.gz -2 SRR2627175_2.fastq.gz --nanopore minion.pass.2D.fastq...2172 days ago
2172 days ago
Comment on "Run miniasm assembler on nanopore reads !"
I followed this to assemble the genome using ONT (nanopore reads) Minimap and miniasm are ultrafast tools for (i) mapping and (ii) assembly. Designed for long, noisy reads, they do no...2172 days ago
Comment on "Does anyone have Nanopore latest updates?"
92% genome coverage of E. coli, average depth of 3.8x. But from just _43_ reads https://github.com/nickloman/massive-nanopore-silliness http://lab.loman.net/2017/03/09/ultrareads-for-nanopore/2550 days ago