Comment on "A quick guide to Phred scaling"
...used system for representing the quality scores of sequencing reads. I...uide to Phred scaling: Phred quality scores: Phred quality scores...ility of error of 1 in 1000. Quality score calculation: The Phred...or storing sequencing reads. Each quality score is represented as an AS...429 days ago
Comment on "Short-read assembly using Spades !"
...ps for short-read assembly using SPAdes: Quality control of raw reads: Before...tant to ensure that the raw reads are of high quality and do not contain sequencing...ning SPAdes, the next step is to evaluate the quality of the assembled genome. This...429 days ago
Comment on "Nextflow: Data-driven computational pipelines"
A collection of high quality Nextflow pipelines https://github.com/nf-core/1550 days ago
Comment on "Important Journals, Blogs and Forums for Bioinformaticians"
NAR: Genomics and Bioinformatics publishes high quality research into genomics and bioinformatics, including novel computational or experimental methods and novel biological findings related to genomics, proteomics, transcriptomics, and epigenetics. https://academic.oup.com/nargab1873 days ago
2113 days ago
Comment on "Running Trinity on RNA-seq !"
...and gene abundance. This is a prerequisite to many other analyses such as examining differentially expressed transcripts among your samples. Quality checking your samples and bio...2137 days ago
2151 days ago
Comment on "GraphMap - A highly sensitive and accurate mapper for long, error-prone reads"
...0.0, thredhold not applied. [1e0] -c, --mapq INT Threshold for mapping quality. If mapq < INT, read will...given fraction of the top score will be counted for ambiguity (mapping quality). Value of 0.0 counts only id...2306 days ago
2813 days ago
Comment on "Software packages for next gen sequence analysis"
MaGuS: a tool for quality assessment and scaffolding of genome assemblies with Whole Genome Profili...d Scaffolding) is a scaffolder and a reference-free evaluator of assembly quality. It uses a draft genome assem...2922 days ago