Protocol for De novo Genome Assembly using Illumina Reads
...hod for small to medium-sized genomes. What is de novo genome assembly?The method of taking...the DNA originated relates to genome assembly. No previous knowled...on a sequencing computer in a genome sequencing experiment. Depend...ew/42542/trimmomatic-command Genome Assembly:The object of this p...1199 days ago
Ancient whole genome duplication (WGD) detection tools !
There are two methods for ancient WGD detection, one is collinearity analysis, and the other is based o.../miniconda3/envs/wgd/make -j 4 make insatall Take the sugarcane genome Saccharum spontaneum L as an example. The genome...1150 days ago
Frequently used bioinformatics tools for viral genome analysis !
IVA: accurate de novo assembly of RNA virus genomes. Hunt M, Gall A, Ong SH, Brener J, Ferns B, Gou...-1 (2012). GAGE: GAGE: A critical evaluation of genome assemblies and assembly algorithms. Salzberg, S. L. et al. Genome...1042 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (f...optional) Alignment-level QC (picard, SAMtools) Genome-wide and amplicon coverage QC...l variant screenshots with annotation tracks (ASCIIGenome)...872 days ago
Useful Bioinformatics Analysis Tools !
CoMeta Classificier of reads from metagenomic sequencing experime...CoMeta: Classification of Metagenomes Using k-mers, PLOS ONE,...Compressor of collections of genome sequences. •  ...obust relative compression of genomes with random access, Bio...858 days ago
List of comparative genomics resources !
3D-GENOMICS -- A Database to Compare Structural and Functional A...species. BGDB -- Bovine Genome Database Find information ab...struction Tool Based on Whole Genomes Construct phylogenetic tree...risons of Multiple Vertebrate Genomes Access to whole genome alig...672 days ago
Online resources on must-read papers in evolutionary biology, for a literature club
1. *Nick Barton:* - The textbook "Evolution" by Nick Barton, with resources fo...09. 1b. Sachidandam et al. 2001. A map of human genome sequence variation containin...ml;bo S., 2010 A draft sequence of the Neandertal genome....672 days ago
Must read paper and books in evolution biology !
1. *Nick Barton:* - The textbook "Evolution" by Nick Barton, with resources fo...09. 1b. Sachidandam et al. 2001. A map of human genome sequence variation containin...ml;bo S., 2010 A draft sequence of the Neandertal genome....572 days ago
Common methods to discover tandem repeats
Tandem repeats are DNA sequences that are repeated in a contiguous manner in the genome. These sequences are often us...ods for discovering tandem repeats in genomes: Tandem Repeat Finder:...ehensively identify tandem repeats in genomes...418 days ago
Common steps for reads mapping !
Mapping reads to a reference genome is an essential step in many types...s to follow for mapping reads to a genome: Choose a read mapper: T...question. Index the reference genome: Before mapping reads, the re...ecifying the input data, reference genome,...418 days ago