Comment on "Calling variants in non-diploid systems"
FreeBayes is widely used for calling variants in diploid systems. However, it can also be used for calling variants in pooled samples where the number of samples is not known. This is the exact sce...1058 days ago
Comment on "Genome in a Bottle (GIAB) Consortium"
...38 under each genome at ftp://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/ Draft benchmar...al duplications). GIAB is also exploring expanding to additional samples consented for release of WGS...1574 days ago
Comment on "Running Trinity on RNA-seq !"
...gene abundance. This is a prerequisite to many other analyses such as examining differentially expressed transcripts among your samples. Quality checking your samples and biological replicates. Ma...2138 days ago
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Comment on "RPKM normalization - Perl"
...sage: perl rpkm_script_beta.pl sample_count_test.count 2:9 28 > sample_count_test.rpkm Colum1 in the input matrix is gene name, column 2 to 9 samples with corresponding counts and...2886 days ago
Comment on "Does anyone have Nanopore latest updates?"
Nano conference https://publications.nanoporetech.com/2016/06/05/london-calling-talk-sara-goodwin-cshl-rapid-cnv-characterisation-of-clinical-cancer-samples-on-the-oxford-nanopore-minion/2905 days ago
Comment on "Next generation sequencing in R or bioconductor environment"
UNDO: a Bioconductor R package for unsupervised deconvolution of mixed gene expressions in tumor samples http://bioinformatics.oxfordjournals.org/content/early/2014/09/10/bioinformatics.btu607.abstract3538 days ago
Comment on "Which are the best statistical programming languages to study for a bioinformatician?"
...;Propensity Score Matching Yes Yes Limited Limited Stratified Samples (Survey Data) Yes...3566 days ago
Comment on "Bioinformatics Algorithm Demonstrations and Tutorials"
A SURVEY OF SEQUENCE MATCHING AND ALIGNMENT ALGORITHMS https://people.ok.ubc.ca/rlawrenc/teaching/404/Project/Samples/Report/SequenceMatching_Report.pdf3844 days ago