support.10xgenomics.com - Supernova generates phased, whole-genome de novo assemblies from a Chromium-prepared library.
Please see Achieving Success with De Novo Assembly and System Requirements before creating your Chromium libraries for...
The School of Biotechnology offers a curriculum that reflects the multidisciplinary nature of Biotechnology, integrating theoretical and applied science in undergraduate and graduate courses. The school has six departments with about 300 employees,...
training.galaxyproject.org - In this tutorial we assemble and annotate the genome of E. coli strain C-1. This strain is routinely used in experimental evolution studies involving bacteriophages. For instance, now classic works by Holly Wichman and Jim Bull (Bull 1997, Bull...
The purpose of this cheat sheet is to introduce biologist and bioinformatician to the frequently used tools for NGS analysis as well as giving experience in writing one-liners.
File System ls — list items in current directory ls...
github.com - IVA (Iterative Virus Assembler) designed specifically for read pairs sequenced at highly variable depth from RNA virus samples. We tested IVA on datasets from 140 sequenced samples from human immunodeficiency virus-1 or influenza-virus-infected...
Reference-free prediction of rearrangement breakpoint reads | Bioinformatics | Oxford Academic
https://academic.oup.com/bioinformatics/article/30/18/2559/2475628 Reference-free SNP detection: dealing with the data...
If we only had Illumina reads, we could also assemble these using the tool Spades.
You can try this here, or try it later on your own data.
Get data
We will use the same Illumina data as we used above:
illumina_R1.fastq.gz: the Illumina...
lh3.github.io - Given a de novo assembly, we often measure the “average” contig length by N50. N50 is neither the real average nor median. It is the length of the contig such that this and longer contigs cover at least 50% of the assembly. A...
These are some of the most famous Interview Puzzles being asked in top tech companies.Here is a list of Top 25 puzzles which have been asked in top Tech Interview.
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