Abhimanyu Singh
A bioinformatics beginner
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Powerful books for learning data analysis with R
By LEGE 178 days agohttps://r-graphics.org/ - R is powerful tool for data analysis, visualization, and machine learning. And it costs $0 to use! Here are six FREE books you can use to learn R...LAMSA: fast split read alignment with long approximate matches
By Jit 2383 days ago Comments (1)github.com - LAMSA (Long Approximate Matches-based Split Aligner) is a novel split alignment approach with faster speed and good ability of handling SV events. It is well-suited to align long reads (over thousands of base-pairs). LAMSA takes takes the...nanofilt: Filtering and trimming of long read sequencing data
By Jit 2307 days ago Comments (1)github.com - Filtering on quality and/or read length, and optional trimming after passing filters.Reads from stdin, writes to stdout. Intended to be used: directly after fastq extraction prior to mapping in a stream between extraction and...rHAT: a seed-and-extension-based noisy long read alignment tool
By Abhimanyu Singh 2252 days agogithub.com - rHAT is a seed-and-extension-based noisy long read alignment tool. It is suitable for aligning 3rd generation sequencing reads which are in large read length with relatively high error rate, especially Pacbio's Single Molecule Read-time (SMRT)...Shasta long read assembler
By Jit 1774 days ago Comments (1)github.com - The goal of the Shasta long read assembler is to rapidly produce accurate assembled sequence using as input DNA reads generated by Oxford Nanopore flow cells. Computational methods used by the Shasta assembler include: Using...PBSuite: Software for Long-Read Sequencing Data from PacBio
By Jit 2825 days agosourceforge.net - PBJelly - the genome upgrading tool. PBHoney - the structural variation discovery tool Both are contained within the PBSuite code found in downloads.----- PBJelly -----Read The...TULIP - The Uncorrected Long read Itegration Pipeline
By Jit 2556 days agogithub.com - #Running TULIP (The Uncorrected Long-read Integration Process), version 0.4 late 2016 (European eel) TULIP currently consists of to Perl scripts, tulipseed.perl and tulipbulb.perl. These are very much intended as prototypes, and additional...LSC: Improving PacBio Long Read Accuracy by Short Read Alignment
By Abhimanyu Singh 2269 days agowww.healthcare.uiowa.edu - Added Command line argument support. Multi-stage execution modes. Support for parallelization. Now execution proceeds in batches of long reads the size of which can be set by --long_read_batch_size N. Better compressed intermediate files. Added...CANU genome assembly parameters !
By Rahul Nayak 2146 days agoChoose the appropriate parameters to run Canu and run it.
Run miniasm assembler on nanopore reads !
Last updated 2531 days ago by Jit Comments (5)Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream...
