BioStar
Bioinformatician
Our Sponsors
Related items
Frequent Paired-end reads (PE 2x100) mapping command lines
Last updated 2385 days ago by Jitbowtie2 -x hs37m -X 650 -q -1 r1.fq -2 r2.fq -S r12.bowtie2.sam bwa aln hs37m.fa r1.fq > r1.sai && bwa aln hs37m.fa r2.fq > r2.sai \ && bwa sampe hs37m r1.sai r2.sai r1.fq r2.fq > r12.bwa.sam bwa bwasw...GenomeMapper: Simultaneous alignment of short reads against multiple genomes
By Jit 2375 days ago1001genomes.org - GenomeMapper is a short read mapping tool designed for accurate read alignments. It quickly aligns millions of reads either with ungapped or gapped alignments. It can be used to align against multiple genomes simulanteously or against a single...ReMILO: reference assisted misassembly detection algorithm using short and long reads.
By Jit 2333 days agogithub.com - ReMILO, a reference assisted misassembly detection algorithm that uses both short reads and PacBio SMRT long reads. ReMILO aligns the initial short reads to both the contigs and reference genome, and then constructs a novel data structure called...SimLoRD: A read simulator for third generation sequencing reads
By Aaryan Lokwani 2286 days agobitbucket.org - SimLoRD is a read simulator for third generation sequencing reads and is currently focused on the Pacific Biosciences SMRT error model. Reads are simulated from both strands of a provided or randomly generated reference sequence. The reference...PANDASEQ is a program to align Illumina reads, optionally with PCR primers embedded in the...
By BioStar 2256 days agogithub.com - Development packages for zlib and libbz2 are needed, as well as a standard compiler environment. On Ubuntu, this can be installed via: sudo apt-get install build-essential libtool automake zlib1g-dev libbz2-dev pkg-config On MacOS, the Apple...Pacasus: Correction of palindromes in long reads from PacBio and Nanopore
By BioStar 2204 days agogithub.com - Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source...FastGT: an alignment-free method for calling common SNVs directly from raw sequencing reads
By Jit 1762 days agobioinfo.ut.ee - FastGT is a program package for whole-genome genotyping of genome variants directly from raw sequencing reads. It is written in C and runs in Linux. FastGT uses a list of variant-specific k-mer pairs that are unique in human genome, counts the...Filtlong: quality filtering tool for long reads
By Radha Agarkar 1656 days agogithub.com - Filtlong is a tool for filtering long reads by quality. It can take a set of long reads and produce a smaller, better subset. It uses both read length (longer is better) and read identity (higher is better) when choosing which reads pass the...Hifiasm: a haplotype-resolved assembler for accurate Hifi reads
By Jit 1431 days agogithub.com - Hifiasm is a fast haplotype-resolved de novo assembler for PacBio Hifi reads. It can assemble a human genome in several hours and works with the California redwood genome, one of the most complex genomes sequenced so far. Hifiasm can produce...Bioinformatics tools for telomere to telomere assembly !
By BioStar 1195 days agooptimize HiFi reads
