BOL

Computational Biology and Bioinformatics in Nigeria https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998874/ #Nigeria #Bioinformatics #Scope

Multi-line fasta to single line fasta $ perl -pe '/^>/ ? print "\n" : chomp' in.fasta | tail -n +2 > out.fasta #Multiline #Singleline #Fasta

Snakemake install Ubuntu $ sudo apt-get install snakemake #snakemake #Ubuntu #Install

#Multiline Fasta To #SingleLine Fasta $ awk '/^>/ {printf("\n%s\n",$0);next; } { printf("%s",$0);} END {printf("\n");}' < file.fa

Long Reads ( pacbio and nanopore) correction https://www.pacb.com/asset_tags/error-correction/ #PacBio #Error #Correction #LongReads

https://scinapse.io/ #Non-profit #academic #search #engine

cat /etc/*-release #System #version #Linux

perl -pe '/^>/ ? print "\n" : chomp' LR.fasta | tail -n +2 > out.fasta #singleLine #Multifasta #Multi2Single

Understanding PairedEnd(PE) mapping https://davetang.org/muse/2014/07/24/getting-started-paired-end-reads/ #PE #Paired #End #PairedEnd #Mapping #Map #Learn

du -sh * | sort | grep "G" #List size of all the directory #Size #List #Directory #Linux

#Remove #smartbell from #pacbio #removesmartbell.sh https://github.com/BioInfoTools/BBMap/blob/master/sh/removesmartbell.sh

Scrubbing long reads https://dazzlerblog.wordpress.com/2017/04/22/1344/ #Scrub #Long #Reads #Pacbio

Genome assembly https://www2.eecs.berkeley.edu/Pubs/TechRpts/2016/EECS-2016-43.pdf #Genome #Assembly #Tutorial

#Canu #assembly of long reads https://canu.readthedocs.io/en/latest/quick-start.html

Long read extraction https://github.com/rrwick/Unicycler/wiki/Read-extraction #Reads #Extractions #Long #PacBio #Ont