TopHat http://tophat.cbcb.umd.edu/manual.shtml is a program that aligns RNA-Seq reads to a genome in order to identify exon-exon splice junctions. It is built on the ultrafast short read mapping program Bowtie.
Hi Sir,
Many of my student and my centre uses your software and tools for project-cum-research work. Thanks for developing such a useful bioinformatics resources. Keep it up.
Thanks
Bioinformatics algorithms at
www.users-birc.au.dk/cstorm/students/Vesterstroem_Mar2006.pdf
www.proteomics.bioengr.uic.edu/people/ezra/cv/thesis_uic.pdf
Hi Ruchira,
It looks confusing to me as well, and question remain WHY? I guess it might be due to parameter differences. I will suggest you to please read this manuscript entitled "A beginner’s guide to eukaryotic genome annotation"...