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Hi Ruchira,           I Think There is Different software using Different Algorithm that's why something result look like different.But u can use GENESCAN. GENESCAN gives accurate result and also used world wide gene...

TopHat http://tophat.cbcb.umd.edu/manual.shtml is a program that aligns RNA-Seq reads to a genome in order to identify exon-exon splice junctions. It is built on the ultrafast short read mapping program Bowtie.

SAMtool quick tutorial at http://davetang.org/wiki/tiki-index.php?page=SAMTools http://samtools.sourceforge.net/samtools.shtml

Hi Sir, Many of my student and my centre uses your software and tools for project-cum-research work. Thanks for developing such a useful bioinformatics resources. Keep it up. Thanks

Bioinformatics algorithms at www.users-birc.au.dk/cstorm/students/Vesterstroem_Mar2006.pdf www.proteomics.bioengr.uic.edu/people/ezra/cv/thesis_uic.pdf