Log in

Our Sponsors



Download BioinformaticsOnline(BOL) Apps in your chrome browser.




Latest activity

  • Neel posted to the wire 2241 days ago
    Free opensource VPN software for mac https://tunnelblick.net/index.html #mac #vpn
  • AnHo published a blog post How to set up SSH on Ubuntu 18.04 2241 days ago
    SSH, also known as Secure Shell or Secure Socket Shell, is a network protocol
    Comments
    • LEGE 1807 days ago

      The SSH server is not installed by default on Ubuntu desktop systems but it can be easily installed from the standard Ubuntu repositories.

      To install and enable SSH on your Ubuntu system complete the following steps:

      Open your terminal either by using the Ctrl+Alt+T keyboard shortcut or by clicking on the terminal icon and install the openssh-server package by typing:

      sudo apt update

      sudo apt install openssh-server

      Once the installation is completed, the SSH service will start automatically. To verify that the installation was successful and SSH service is running type the following command which will print the SSH server status:

      sudo systemctl status ssh

      Ubuntu comes with a firewall configuration tool called UFW. If the firewall is enabled on your system, make sure to open the SSH port:

      sudo ufw allow ssh

      Once you’ve found the IP address, login to remote machine by running the following ssh command:

      ssh user@ip

      If you don’t know your IP address you can easily find it using the this

      ip a
  • AnHo is now a friend with Jitendra Narayan 2241 days ago
  • Neel published a blog post List of motif discovery tools ! 2244 days ago
    A DNA sequence motif represented as a sequence logo for the LexA-binding motif. I
    Comments
  • The Kubeflow project is dedicated to making deployments of machine learning (ML) workflows on Kubernetes simple, portable and scalable. Our goal is not to recreate other services, but to provide a straightforward way to deploy best-of-breed...
  • BioStar posted to the wire 2248 days ago
  • NCBI Hackathon are pleased to announce the second installment of the SoCal Bioinformatics Hackathon. From January 9-11, 2019, the NCBI will help run a bioinformatics hackathon in Southern California hosted by the Computational Sciences Research...
  • NovoGraph: building whole genome graphs from long-read-based de novo assemblies An algorithmically novel approach to construct a genome graph representation of long-read-based de novo sequence assemblies. We then provide a proof of...
  • Abhimanyu Singh posted to the wire 2248 days ago
    #Intersect your bed data with coverage $ bedtools genomecov -ibam aln.bam -bga | awk '$4==0' | bedtools intersect -a cds.bed -b - -f 1.0
  • Rahul Nayak posted to the wire 2249 days ago
    > pchisq(15, df=2, lower.tail=FALSE); [1] 0.0005530844 #Chi-square #R #P-value
  • Given the potential relevance of efficient haplotyping in several analysis pipelines, we have designed and engineered pWhatsHap, a parallel, high-performance version of WhatsHap. pWhatsHap is embedded in a toolkit developed in...
  • MEGAHIT is a single node assembler for large and complex metagenomics NGS reads, such as soil. It makes use of succinct de Bruijn graph (SdBG) to achieve low memory assembly. MEGAHIT can optionally utilize a CUDA-enabled GPU to...
  • SKESA is a DeBruijn graph-based de-novo assembler designed for assembling reads of microbial genomes sequenced using Illumina. Comparison with SPAdes and MegaHit shows that SKESA produces assemblies that have high sequence quality and contiguity,...
  • ANItools is a software package written by PERL scripts that can be run in a Linux/Unix system. If you want to compare bacterial genomes and calculate their average nucleotide identity (ANI), you could download and run this program directly. Or you...
  • Required python modules biopython intervaltree numpy scipy tqdm PyYAML
  • Bioinformatics jobs in Gulf https://www.naukrigulf.com/bioinformatics-jobs
  • Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source...
  • ASCIIGenome is a genome browser based on command line interface and designed for running from console terminals. Since ASCIIGenome does not require a graphical interface it is particularly useful for quickly visualizing genomic data...
    Comments
    • Jit 1771 days ago

      This a list of available commands with their brief description.                                                               
      For help on individual commands use one of::                                                                                  
                                                                                                                                    
          command -h                                                                                                                
          ?command                                                                                                                  
          help command                                                                                                              
                                                                                                                                    
      e.g. `ylim -h`                                                                                                                
                                                                                                                                    
            N a v i g a t i o n                                                                                                     
                                                                                                                                    
      goto chrom:[from]-[to]                                                                                                        
            Go to region `chrom:from-to` or to `chrom:from` or to the start of                                                      
            `chrom`.                                                                                                                
      INT from [c | to]                                                                                                             
            Go to position `from` or to region `from to` on current chromosome.                                                     
      PERCENT from [c | to]                                                                                                         
            Zoom into the current window delimited by given PERCENT of screen.                                                      
      + INT [k|m]                                                                                                                   
            Move forward by `INT` bases. Suffix K/M recognized.                                                                     
      - INT [k|m]                                                                                                                   
            Move backwards by INT bases. Suffix K/M recognized.                                                                     
      f [NUM=0.1]                                                                                                                   
            Move forward NUM times the size of the current window, 1/10 by default.                                                 
      b [NUM=0.1]                                                                                                                   
            Move backward NUM times the size of the current window, 1/10 by default                                                 
      ff                                                                                                                            
            Move forward by 1/2 of a window. A shortcut for `f 0.5`                                                                 
      bb                                                                                                                            
            Move backward by 1/2 of a window. A shortcut for `b 0.5`                                                                
      zi [INT = 1]                                                                                                                  
            Zoom in INT times. Each zoom halves the window size.                                                                    
      zo [INT = 1]                                                                                                                  
            Zoom out INT times. Each zoom doubles the window size.                                                                  
      extend [mid|window] [INT left] [INT right]                                                                                    
            Extend the current window by `INT` bases left and right.                                                                
      l                                                                                                                             
            Go to the Left half of the current window.                                                                              
      r                                                                                                                             
            Go to the Right half of the current window.                                                                             
      p                                                                                                                             
            Go to the previous visited position.                                                                                    
      n                                                                                                                             
            Go to the next visited position.                                                                                        
      next [-back] [-start] [-zo INT=5] [track]                                                                                     
            Move to the next feature not overlapping the current coordinates.                                                       
                                                                                                                                    
            F i n d                                                                                                                 
                                                                                                                                    
      find [-all] [-c] [-F] regex [track]                                                                                           
            Find the first record in `track` containing `regex`.                                                                    
      seqRegex [-iupac] [-c] [regex]                                                                                                
            Find regex in reference sequence and show matches as an additional                                                      
            track.                                                                                                                  
      bookmark [-d] [-n name] [-print] [> file] [chrom:from-to]                                                                     
            Creates a track to save positions of interest.                                                                          
                                                                                                                                    
            D i s p l a y                                                                                                           
                                                                                                                                    
      grep [-i = .*] [-e = ''] [-c] [-F] [-v] [track_regex = .*]...                                                                 
            Similar to grep command, filter for features including or excluding                                                     
            patterns.                                                                                                               
      awk [-off ...] [-F sep_re] [-v VAR=var] [-V] '<script>' [track_regex = .*]...                                                 
            Advanced feature filtering using awk syntax.                                                                            
      featureColorForRegex [-r/-R regex color] [-v] [track_regex = .*]...                                                           
            Set colour for features captured by regex.                                                                              
      featureDisplayMode [-expanded | -collapsed | -oneline] [-v] [track_regex = .*]...                                             
            Set how annotation features should be displayed.                                                                        
      gap [-on | -off] [-v] [track_regex = .*]...                                                                                   
            Display features with or without a separating gap.                                                                      
      gffNameAttr [attribute_name = NULL | -na] [-v] [track_regex = .*]...                                                          
            GTF/GFF attribute to set the feature name or `-na` to suppress name.                                                    
      trackHeight [-v] INT [track_regex = .*]...                                                                                    
            Set track height to INT lines of text for all tracks matching regexes.                                                  
      ylim [-v] <NUM|min|na> <NUM|min|na> [track_regex = .*]...                                                                     
            Set the y-axis limit for all tracks matched by regexes.                                                                 
      colorTrack [-v] color [track_regex = .*]...                                                                                   
            Set colour for tracks matched by regex.                                                                                 
      hideTitle [-on | -off] [-v] [track_regex = .*]...                                                                             
            Set the display of the title line matched by track_regex.                                                               
      genotype [-n 10] [-s .*] [-r pattern rplc] [-f expr] [-v] [track_regex = .*]...                                               
            Customise the genotype rows printed under the VCF tracks.                                                               
      editNames [-t] [-v] <pattern> <replacement> [track_re=.*]...                                                                  
            Edit track names by substituting regex pattern with replacement.                                                        
      dataCol [-v] [index = 4] [track_regex = .*]...                                                                                
            Select data column for bedgraph tracks containing regex.                                                                
      print [-n INT] [-full] [-off] [-round INT] [-hl re] [-esf] [-v] [-sys CMD] [track_regex = .*]... [>|>> file]                  
            Print lines for the tracks matched by `track_regex`.                                                                    
                                                                                                                                    
            A l i g n m e n t s                                                                                                     
                                                                                                                                    
      readsAsPairs [-on | -off] [-v] [track_regex = .*]...                                                                          
            Show SAM records as pairs.                                                                                              
      filterVariantReads [-r from/to] [-all] [-v] [track_regex = .*]...                                                             
            Filter reads containing a variant in the given interval.                                                                
      rpm [-on | -off] [-v] [track_regex = .*]                                                                                      
            Set display to reads per million for BAM and TDF files.                                                                 
      samtools [-f INT=0] [-F INT=4] [-q INT=0] [-v] [track_re = .*] ...                                                            
            Apply samtools filters to alignment tracks captured by the list of                                                      
            track regexes.                                                                                                          
      BSseq [-on | -off] [-v] [track_regex = .*]...                                                                                 
            Set bisulfite mode for read tracks matched by regex.                                                                    
                                                                                                                                    
            G e n e r a l                                                                                                           
                                                                                                                                    
      setGenome fasta|bam|genome                                                                                                    
            Set genome and reference sequence.                                                                                      
      setConfig <file|tag> | <key> <value>                                                                                          
            Set configuration arguments.                                                                                            
      explainSamFlag INT [INT ...]                                                                                                  
            Explain the list of bitwise SAM flags.                                                                                  
      show <arg>                                                                                                                    
            Show or set features to display.                                                                                        
      recentlyOpened [-grep = .*]                                                                                                   
            List recently opened files.                                           &nbsp

    • Rahul Nayak 1771 days ago

      Install ASCIIGenome with conda

      (base) [wsu29@bladeamd-2 ~]$ conda install ASCIIGenome
      Collecting package metadata (current_repodata.json): done
      Solving environment: done

      ## Package Plan ##

        environment location: /data/sata_data/workshop/wsu29/miniconda3

        added / updated specs:
          - asciigenome


      The following packages will be downloaded:

          package                    |            build
          ---------------------------|-----------------
          asciigenome-1.15.0         |                0        27.5 MB  bioconda
          openjdk-8.0.152            |       h7b6447c_3        57.4 MB
          ------------------------------------------------------------
                                                 Total:        84.9 MB

      The following NEW packages will be INSTALLED:

        asciigenome        bioconda/noarch::asciigenome-1.15.0-0
        openjdk            pkgs/main/linux-64::openjdk-8.0.152-h7b6447c_3


      Proceed ([y]/n)? y


      Downloading and Extracting Packages
      openjdk-8.0.152      | 57.4 MB   | ################################################################################### | 100%
      asciigenome-1.15.0   | 27.5 MB   | ################################################################################### | 100%
      Preparing transaction: done
      Verifying transaction: done
      Executing transaction: done