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  • SKESA is a DeBruijn graph-based de-novo assembler designed for assembling reads of microbial genomes sequenced using Illumina. Comparison with SPAdes and MegaHit shows that SKESA produces assemblies that have high sequence quality and contiguity,...
  • ANItools is a software package written by PERL scripts that can be run in a Linux/Unix system. If you want to compare bacterial genomes and calculate their average nucleotide identity (ANI), you could download and run this program directly. Or you...
  • Required python modules biopython intervaltree numpy scipy tqdm PyYAML
  • Bioinformatics jobs in Gulf https://www.naukrigulf.com/bioinformatics-jobs
  • Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source...
  • ASCIIGenome is a genome browser based on command line interface and designed for running from console terminals. Since ASCIIGenome does not require a graphical interface it is particularly useful for quickly visualizing genomic data...
    Comments
    • Jit 1723 days ago

      This a list of available commands with their brief description.                                                               
      For help on individual commands use one of::                                                                                  
                                                                                                                                    
          command -h                                                                                                                
          ?command                                                                                                                  
          help command                                                                                                              
                                                                                                                                    
      e.g. `ylim -h`                                                                                                                
                                                                                                                                    
            N a v i g a t i o n                                                                                                     
                                                                                                                                    
      goto chrom:[from]-[to]                                                                                                        
            Go to region `chrom:from-to` or to `chrom:from` or to the start of                                                      
            `chrom`.                                                                                                                
      INT from [c | to]                                                                                                             
            Go to position `from` or to region `from to` on current chromosome.                                                     
      PERCENT from [c | to]                                                                                                         
            Zoom into the current window delimited by given PERCENT of screen.                                                      
      + INT [k|m]                                                                                                                   
            Move forward by `INT` bases. Suffix K/M recognized.                                                                     
      - INT [k|m]                                                                                                                   
            Move backwards by INT bases. Suffix K/M recognized.                                                                     
      f [NUM=0.1]                                                                                                                   
            Move forward NUM times the size of the current window, 1/10 by default.                                                 
      b [NUM=0.1]                                                                                                                   
            Move backward NUM times the size of the current window, 1/10 by default                                                 
      ff                                                                                                                            
            Move forward by 1/2 of a window. A shortcut for `f 0.5`                                                                 
      bb                                                                                                                            
            Move backward by 1/2 of a window. A shortcut for `b 0.5`                                                                
      zi [INT = 1]                                                                                                                  
            Zoom in INT times. Each zoom halves the window size.                                                                    
      zo [INT = 1]                                                                                                                  
            Zoom out INT times. Each zoom doubles the window size.                                                                  
      extend [mid|window] [INT left] [INT right]                                                                                    
            Extend the current window by `INT` bases left and right.                                                                
      l                                                                                                                             
            Go to the Left half of the current window.                                                                              
      r                                                                                                                             
            Go to the Right half of the current window.                                                                             
      p                                                                                                                             
            Go to the previous visited position.                                                                                    
      n                                                                                                                             
            Go to the next visited position.                                                                                        
      next [-back] [-start] [-zo INT=5] [track]                                                                                     
            Move to the next feature not overlapping the current coordinates.                                                       
                                                                                                                                    
            F i n d                                                                                                                 
                                                                                                                                    
      find [-all] [-c] [-F] regex [track]                                                                                           
            Find the first record in `track` containing `regex`.                                                                    
      seqRegex [-iupac] [-c] [regex]                                                                                                
            Find regex in reference sequence and show matches as an additional                                                      
            track.                                                                                                                  
      bookmark [-d] [-n name] [-print] [> file] [chrom:from-to]                                                                     
            Creates a track to save positions of interest.                                                                          
                                                                                                                                    
            D i s p l a y                                                                                                           
                                                                                                                                    
      grep [-i = .*] [-e = ''] [-c] [-F] [-v] [track_regex = .*]...                                                                 
            Similar to grep command, filter for features including or excluding                                                     
            patterns.                                                                                                               
      awk [-off ...] [-F sep_re] [-v VAR=var] [-V] '<script>' [track_regex = .*]...                                                 
            Advanced feature filtering using awk syntax.                                                                            
      featureColorForRegex [-r/-R regex color] [-v] [track_regex = .*]...                                                           
            Set colour for features captured by regex.                                                                              
      featureDisplayMode [-expanded | -collapsed | -oneline] [-v] [track_regex = .*]...                                             
            Set how annotation features should be displayed.                                                                        
      gap [-on | -off] [-v] [track_regex = .*]...                                                                                   
            Display features with or without a separating gap.                                                                      
      gffNameAttr [attribute_name = NULL | -na] [-v] [track_regex = .*]...                                                          
            GTF/GFF attribute to set the feature name or `-na` to suppress name.                                                    
      trackHeight [-v] INT [track_regex = .*]...                                                                                    
            Set track height to INT lines of text for all tracks matching regexes.                                                  
      ylim [-v] <NUM|min|na> <NUM|min|na> [track_regex = .*]...                                                                     
            Set the y-axis limit for all tracks matched by regexes.                                                                 
      colorTrack [-v] color [track_regex = .*]...                                                                                   
            Set colour for tracks matched by regex.                                                                                 
      hideTitle [-on | -off] [-v] [track_regex = .*]...                                                                             
            Set the display of the title line matched by track_regex.                                                               
      genotype [-n 10] [-s .*] [-r pattern rplc] [-f expr] [-v] [track_regex = .*]...                                               
            Customise the genotype rows printed under the VCF tracks.                                                               
      editNames [-t] [-v] <pattern> <replacement> [track_re=.*]...                                                                  
            Edit track names by substituting regex pattern with replacement.                                                        
      dataCol [-v] [index = 4] [track_regex = .*]...                                                                                
            Select data column for bedgraph tracks containing regex.                                                                
      print [-n INT] [-full] [-off] [-round INT] [-hl re] [-esf] [-v] [-sys CMD] [track_regex = .*]... [>|>> file]                  
            Print lines for the tracks matched by `track_regex`.                                                                    
                                                                                                                                    
            A l i g n m e n t s                                                                                                     
                                                                                                                                    
      readsAsPairs [-on | -off] [-v] [track_regex = .*]...                                                                          
            Show SAM records as pairs.                                                                                              
      filterVariantReads [-r from/to] [-all] [-v] [track_regex = .*]...                                                             
            Filter reads containing a variant in the given interval.                                                                
      rpm [-on | -off] [-v] [track_regex = .*]                                                                                      
            Set display to reads per million for BAM and TDF files.                                                                 
      samtools [-f INT=0] [-F INT=4] [-q INT=0] [-v] [track_re = .*] ...                                                            
            Apply samtools filters to alignment tracks captured by the list of                                                      
            track regexes.                                                                                                          
      BSseq [-on | -off] [-v] [track_regex = .*]...                                                                                 
            Set bisulfite mode for read tracks matched by regex.                                                                    
                                                                                                                                    
            G e n e r a l                                                                                                           
                                                                                                                                    
      setGenome fasta|bam|genome                                                                                                    
            Set genome and reference sequence.                                                                                      
      setConfig <file|tag> | <key> <value>                                                                                          
            Set configuration arguments.                                                                                            
      explainSamFlag INT [INT ...]                                                                                                  
            Explain the list of bitwise SAM flags.                                                                                  
      show <arg>                                                                                                                    
            Show or set features to display.                                                                                        
      recentlyOpened [-grep = .*]                                                                                                   
            List recently opened files.                                           &nbsp

    • Rahul Nayak 1723 days ago

      Install ASCIIGenome with conda

      (base) [wsu29@bladeamd-2 ~]$ conda install ASCIIGenome
      Collecting package metadata (current_repodata.json): done
      Solving environment: done

      ## Package Plan ##

        environment location: /data/sata_data/workshop/wsu29/miniconda3

        added / updated specs:
          - asciigenome


      The following packages will be downloaded:

          package                    |            build
          ---------------------------|-----------------
          asciigenome-1.15.0         |                0        27.5 MB  bioconda
          openjdk-8.0.152            |       h7b6447c_3        57.4 MB
          ------------------------------------------------------------
                                                 Total:        84.9 MB

      The following NEW packages will be INSTALLED:

        asciigenome        bioconda/noarch::asciigenome-1.15.0-0
        openjdk            pkgs/main/linux-64::openjdk-8.0.152-h7b6447c_3


      Proceed ([y]/n)? y


      Downloading and Extracting Packages
      openjdk-8.0.152      | 57.4 MB   | ################################################################################### | 100%
      asciigenome-1.15.0   | 27.5 MB   | ################################################################################### | 100%
      Preparing transaction: done
      Verifying transaction: done
      Executing transaction: done

  • To run the application on UNIX from the downloaded jar file run the UNIX: java -mx512m -jar BamView.jar and extra command line options are given when '-h' is used: java -jar BamView.jar -h BAM files can be specified on the command line with the...
  • SAMStat is an efficient C program to quickly display statistics of large sequence files from next generation sequencing projects. When applied to SAM/BAM files all statistics are reported for unmapped, poorly and accurately mapped reads...
  • pyGenomeTracks aims to produce high-quality genome browser tracks that are highly customizable. Currently, it is possible to plot: bigwig bed (many options) bedgraph links (represented as arcs) Hi-C matrices (if HiCExplorer is...
  • Neel created a new bio-script Script to Plot the Coverage 2207 days ago
  • Neel created a new bio-script Collecting arguments with R 2207 days ago
  • An increasing number of studies are mapping protein binding and nucleotide modifications sites throughout the transcriptome. Often, these sites cluster in certain regions of the transcript, giving clues to their function. Hence, it is informative to...
  • Modern genome sequencing technologies provide a succint measure of quality at each position in every read, however all of this information is lost in the assembly process. Referee summarizes the quality information from the reads that map to a site...
  • https://www.illumina.com/company/news-center/press-releases/2018/2374913.html
  • Jit posted to the wire 2214 days ago
    Rename the fasta header perl -ane 'if(/\>/){$a++;print ">LR$a\n"}else{print;}' allPacBio_clean.fa > allPacBio_clean_shortName.fa #fasta #header #rename
  •  Ancestral Genomes (http://ancestralgenomes.org) is a resource for comprehensive reconstructions of these ‘fossil genomes’. Comprehensive sets of protein-coding genes have been reconstructed for 78 genomes of now-extinct species...