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- Abhi 788 days ago
Sequencing coverage is defined as the average number of reads that covers each base of the reference genome. Estimating the sequencing coverage is very important when you are simulating datasets. The coverage equation is defined as follows.
C = LN / G
- C is the sequencing coverage
- G is the length of the genome
- L is the read length
- N is the number of reads
For example, if you have a genome of length 5Mbp and you simulate 1,000,000 HiSeq 2000 reads (read length is 100bp), then we will get a sequencing coverage of
20xas follows.C = LN / G = 100 * 1,000,000 / 5,000,000 = 20x
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