BOL

E-value & Bit-score http://www.metagenomics.wiki/tools/blast/evalue #E-value #Bit-score #blast #Score

Blast database https://ftp.ncbi.nih.gov/blast/db/ #blast #database

Alignment algorithms http://vlab.amrita.edu/?sub=3&brch=274&sim=1431&cnt=1 #Alignments #Algo #Blast

#tBLASTn #command line $ tblastn -query Avaga.pep.out.45.fa -db myDB -outfmt 6 -out output_file -evalue 1e-5 -max_target_seqs 1 #Blast #Protein

http://papers.genomics.lbl.gov/cgi-bin/litSearch.cgi #Publication #blast

Understanding BLAST scores http://homepages.ulb.ac.be/~dgonze/TEACHING/stat_scores.pdf #blast #score

Understanding BitScore https://www.ctu.edu.vn/~dvxe/Bioinformatic%20course/lectures/scores.pdf #Blast #Bit #BitScore

#Target 4 and #HSPS 1 with #blast $ blastn -task megablast -query brkSeq.fa -db myDBAvaga -evalue 1e-5 -num_threads 1 -max_target_seqs 4 -max_hsps 1 -outfmt '6' -out output

Understanding MegaBlast https://gordonlab.wustl.edu/btheta/blast/docs/megablast_readme.html #Blast #BLASTn #MegaBlast #Alignment

BLAST cheats heat http://www.vicbioinformatics.com/documents/Quick_Start_Guide_BLAST_to_BLAST+.pdf #BLAST #NCBI #Cheat

Update NCBI BLAST database in your local Linux system $ update_blastdb --passive --decompress nr nt #BLAST #Updates #NT #NR #Linux

Web BLAST ftp://ftp.ncbi.nlm.nih.gov/blast/documents/web_blast.pl #BLAST #Web #Online

#Local #BLAST tutorial http://www.woolfit.net/perl/51blastall.html

The BLAST parser http://kirill-kryukov.com/study/tools/blast-parser/ #BLAST #Parser #Genome #Match #Sequence #Similarity