With kallisto | bustools you can
Generate a cell x gene or cell x transcript equivalence class count matrix
Perform RNA velocity and single-nuclei RNA-seq analsis
Quantify data from numerous technologies such as...
ComplexHeatmap (Gu, Eils, and Schlesner (2016)) is an R Programming Language (R Core Team (2020)) package that is currently listed in the Bioconductor package repository.
install and load required packages...
Essential genes are those indispensable for the survival of an organism, and their functions are therefore considered a foundation of life. Determination of a minimal gene set needed to sustain a life form, a fundamental question in biology, plays a...
Essential genes are those indispensable for the survival of an organism, and their functions are therefore considered a foundation of life. Determination of a minimal gene set needed to sustain a life form, a fundamental question in biology, plays a...
(base) lege@Bathymodiolus:~$ ./alienomics_v0.3.1Can't load '/tmp/par-6a6974656e647261/cache-f3e57c2becbb7e00629c1273cf03dd05ad45721b/5b8c5ac2.so' for module POSIX: /lib/x86_64-linux-gnu/libm.so.6: version `GLIBC_2.23' not found (required by...
Multi fasta to single fasta files $ perl -ne 'if (/^>(\S+)/) { close OUT; open OUT, ">$1.fasta" } print OUT' genome.fasta #Multi-fasta #fasta#multi#genome#files
https://www.nature.com/articles/s41467-020-17155-yServer Infrastructure:
File Server:
dhara: Synology 3614 Storage Appliance
4 Core Xeon
108TB disk storage
10Gb ethernet to SCG3
Access atx: dhara:5000
Has btsync server (try it - its much...
Janggu is a python package that facilitates deep learning in the context of genomics. The package is freely available under a GPL-3.0 license.
Detail tutorial at https://janggu.readthedocs.io/en/latest/
USE...